Antibody Name:	Anti-RPS14 Antibody
Antibody SKU:	CAB6727
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-151 of human RPS14 (NP_005608.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, SW480, HeLa, HepG2, Jurkat, Mouse spleen, Mouse lung, Rat spleen, HL-60

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-151 of human RPS14 (NP_005608.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAPR KGKE KKEE QVIS LGPQ VAEG ENVF GVCH IFAS FNDT FVHV TDLS GKET ICRV TGGM KVKA DRDE SSPY AAML AAQD VAQR CKEL GITA LHIK LRAT GGNR TKTP GPGA QSAL RALA RSGM KIGR IEDV TPIP SDST RRKG GRRG RRL
Gene ID:	6208
Uniprot:	P62263
Cellular Location:
Calculated MW:	16kDa
Observed MW:	16kDa

Synonyms:

RPS14, EMTB, S14

Background:

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S11P family of ribosomal proteins. It is located in the cytoplasm. Transcript variants utilizing alternative transcription initiation sites have been described in the literature. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. In Chinese hamster ovary cells, mutations in this gene can lead to resistance to emetine, a protein synthesis inhibitor. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.

UniProt Protein Function:	RPS14: a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S11P family of ribosomal proteins. It is located in the cytoplasm. Transcript variants utilizing alternative transcription initiation sites have been described in the literature. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. In Chinese hamster ovary cells, mutations in this gene can lead to resistance to emetine, a protein synthesis inhibitor. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Protein Details:	Protein type:RNA-binding; Ribosomal; Translation Chromosomal Location of Human Ortholog: 5q31-q33 Cellular Component: focal adhesion; membrane; mitochondrion; cytoplasm; nucleolus; cytosol Molecular Function:structural constituent of ribosome; RNA binding; translation regulator activity; mRNA 5'-UTR binding Biological Process: SRP-dependent cotranslational protein targeting to membrane; viral reproduction; translation; viral infectious cycle; negative regulation of transcription from RNA polymerase II promoter; translational termination; maturation of SSU-rRNA; regulation of translation; cellular protein metabolic process; translational elongation; translational initiation; mRNA catabolic process, nonsense-mediated decay; ribosomal small subunit assembly and maintenance; erythrocyte differentiation; gene expression; viral transcription Disease: Chromosome 5q Deletion Syndrome
NCBI Summary:	Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S11P family of ribosomal proteins. It is located in the cytoplasm. Transcript variants utilizing alternative transcription initiation sites have been described in the literature. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. In Chinese hamster ovary cells, mutations in this gene can lead to resistance to emetine, a protein synthesis inhibitor. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	P62263
NCBI GenInfo Identifier:	50403752
NCBI Gene ID:	6208
NCBI Accession:	P62263.3
UniProt Secondary Accession:	P62263,P06366, Q5BJI0, B2R5G5, D3DQG5,
UniProt Related Accession:	P62263
Molecular Weight:	151
NCBI Full Name:	40S ribosomal protein S14
NCBI Synonym Full Names:	ribosomal protein S14
NCBI Official Symbol:	RPS14
NCBI Official Synonym Symbols:	S14; EMTB
NCBI Protein Information:	40S ribosomal protein S14; emetine resistance
UniProt Protein Name:	40S ribosomal protein S14
Protein Family:	Ribosomal protein
UniProt Gene Name:	RPS14
UniProt Entry Name:	RS14_HUMAN

	Western blot analysis of extracts of various cell lines, using RPS14 antibody (CAB6727) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat pancreas using RPS14 Rabbit pAb (CAB6727) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse spinal cord using RPS14 Rabbit pAb (CAB6727) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of MCF7 cells using RPS14 antibody (CAB6727). Blue: DAPI for nuclear staining.