Antibody Name:	Anti-RPS12 Antibody
Antibody SKU:	CAB5890
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-132 of human RPS12 (NP_001007.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, MCF7, HepG2, Raji, NIH/3T3, Mouse lung, Mouse spleen, Mouse thymus

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-132 of human RPS12 (NP_001007.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAEE GIAA GGVM DVNT ALQE VLKT ALIH DGLA RGIR EAAK ALDK RQAH LCVL ASNC DEPM YVKL VEAL CAEH QINL IKVD DNKK LGEW VGLC KIDR EGKP RKVV GCSC VVVK DYGK ESQA KDVI EEYF KCKK
Gene ID:	6206
Uniprot:	P25398
Cellular Location:	Cytoplasm
Calculated MW:	14kDa
Observed MW:	15kDa

Synonyms:

RPS12, S12

Background:

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S12E family of ribosomal proteins. It is located in the cytoplasm. Increased expression of this gene in colorectal cancers compared to matched normal colonic mucosa has been observed. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

UniProt Protein Function:	RPS12: a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S12E family of ribosomal proteins. It is located in the cytoplasm. Increased expression of this gene in colorectal cancers compared to matched normal colonic mucosa has been observed. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
UniProt Protein Details:	Protein type:Translation Chromosomal Location of Human Ortholog: 6q23.2 Cellular Component: cytosol; membrane Molecular Function:structural constituent of ribosome Biological Process: cellular protein metabolic process; gene expression; mRNA catabolic process, nonsense-mediated decay; selenium metabolic process; selenocysteine metabolic process; SRP-dependent cotranslational protein targeting to membrane; translation; translational elongation; translational initiation; translational termination; viral infectious cycle; viral reproduction; viral transcription
NCBI Summary:	Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S12E family of ribosomal proteins. It is located in the cytoplasm. Increased expression of this gene in colorectal cancers compared to matched normal colonic mucosa has been observed. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
UniProt Code:	P25398
NCBI GenInfo Identifier:	224471878
NCBI Gene ID:	6206
NCBI Accession:	P25398.3
UniProt Secondary Accession:	P25398,Q76M58,
UniProt Related Accession:	P25398
Molecular Weight:	14,515 Da
NCBI Full Name:	40S ribosomal protein S12
NCBI Synonym Full Names:	ribosomal protein S12
NCBI Official Symbol:	RPS12
NCBI Official Synonym Symbols:	S12
NCBI Protein Information:	40S ribosomal protein S12
UniProt Protein Name:	40S ribosomal protein S12
Protein Family:	Ribosomal protein
UniProt Gene Name:	RPS12
UniProt Entry Name:	RS12_HUMAN

	Western blot analysis of extracts of various cell lines, using RPS12 antibody (CAB5890) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
	Immunohistochemistry of paraffin-embedded rat brain using RPS12 antibody (CAB5890) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human breast using RPS12 antibody (CAB5890) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using RPS12 antibody (CAB5890) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of MCF-7 cells using RPS12 antibody (CAB5890). Blue: DAPI for nuclear staining.