Epigenetics & Nuclear Signaling Antibodies 1

Anti-RPRD1B Antibody (CAB10102)

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SKU:
CAB10102
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-RPRD1B Antibody
Antibody SKU:CAB10102
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human rprd1b
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:MCF7, SKOV3, NCI-H460, Mouse liver, Mouse lung, Mouse stomach, Mouse spleen, Rat liver
Immunogen:Recombinant protein of human rprd1b
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:58490
Uniprot:Q9NQG5
Cellular Location:Nucleus
Calculated MW:36kDa
Observed MW:37kDa
Synonyms:RPRD1B, C20orf77, CREPT, NET60, dJ1057B20.2, K-H, Kub5-Hera, rprd1b
Background:
UniProt Protein Function:RPRD1B: Interacts with phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and participates in dephosphorylation of the CTD. Transcriptional regulator which enhances expression of CCND1. Promotes binding of RNA polymerase II to the CCDN1 promoter and to the termination region before the poly-A site but decreases its binding after the poly-A site. Prevents RNA polymerase II from reading through the 3' end termination site and may allow it to be recruited back to the promoter through promotion of the formation of a chromatin loop. Also enhances the transcription of a number of other cell cycle-related genes including CDK2, CDK4, CDK6 and cyclin-E but not CDKN1A, CDKN1B or cyclin-A. Promotes cell proliferation. Belongs to the UPF0400 (RTT103) family.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 20q11.23

Cellular Component: centrosome; cytosol; DNA-directed RNA polymerase II, holoenzyme; nucleoplasm; nucleus

Molecular Function:protein binding

Biological Process: positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; snRNA transcription from RNA polymerase II promoter

UniProt Code:Q9NQG5
NCBI GenInfo Identifier:23813907
NCBI Gene ID:58490
NCBI Accession:Q9NQG5.1
UniProt Secondary Accession:Q9NQG5,Q1WDE7, Q6PKF4,
UniProt Related Accession:Q9NQG5
Molecular Weight:37kDa
NCBI Full Name:Regulation of nuclear pre-mRNA domain-containing protein 1B
NCBI Synonym Full Names:regulation of nuclear pre-mRNA domain containing 1B
NCBI Official Symbol:RPRD1B  
NCBI Official Synonym Symbols:CREPT; NET60; C20orf77; dJ1057B20.2  
NCBI Protein Information:regulation of nuclear pre-mRNA domain-containing protein 1B
UniProt Protein Name:Regulation of nuclear pre-mRNA domain-containing protein 1B
UniProt Synonym Protein Names:Cell cycle-related and expression-elevated protein in tumor
Protein Family:Regulation of nuclear pre-mRNA domain-containing protein
UniProt Gene Name:RPRD1B  
Anti-RPRD1B Antibody (CAB10102)Western blot analysis of extracts of various cell lines, using rprd1b antibody (CAB10102) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
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