Cell Biology Antibodies 6
Anti-RPRD1A Antibody (CAB15854)
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- SKU:
- CAB15854
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-RPRD1A Antibody |
| Antibody SKU: | CAB15854 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 47-276 of human RPRD1A (NP_001290341.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HT-29, HeLa, A-431, Mouse liver, Mouse testis, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 47-276 of human RPRD1A (NP_001290341.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | PVIV EAFK HVSS ETDE SCKK HLGR VLSI WEER SVYE NDVL EQLK QALY GDKK PRKR TYEQ IKVD ENEN CSSL GSPS EPPQ TLDL VRAL QDLE NAAS GDAA VHQR IASL PVEV QEVS LLDK ITDK ESGE RLSK MVED ACML LADY NGRL AAEI DDRK QLTR MLAD FLRC QKEA LAEK EHKL EEYK RKLA RVSL VRKE LRSR IQSL PDLS RLPN VTGS HMHL PFAG DIYS ED |
| Gene ID: | 55197 |
| Uniprot: | Q96P16 |
| Cellular Location: | |
| Calculated MW: | 31kDa/35kDa |
| Observed MW: | 36kDa |
| Synonyms: | RPRD1A, HsT3101, P15RS |
| Background: | This gene encodes a cell-cycle and transcription regulatory protein. The encoded protein interacts with the cell cycle inhibitor cyclin-dependent kinase 4 inhibitor B and may function as a negative regulator of G(1)/S phase progression. This protein also forms homo- and hetrodimers with the protein, regulation of nuclear pre-mRNA domain-containing protein 1B, to form a scaffold that interacts with the C-terminal domain of RNA polymerase II subunit B1 and regulates several aspects of transcription. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 16. |
| UniProt Protein Function: | Interacts with phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and participates in dephosphorylation of the CTD by RPAP2. May act as a negative regulator of cyclin-D1 (CCND1) and cyclin-E (CCNE1) in the cell cycle. |
| NCBI Summary: | This gene encodes a cell-cycle and transcription regulatory protein. The encoded protein interacts with the cell cycle inhibitor cyclin-dependent kinase 4 inhibitor B and may function as a negative regulator of G(1)/S phase progression. This protein also forms homo- and hetrodimers with the protein, regulation of nuclear pre-mRNA domain-containing protein 1B, to form a scaffold that interacts with the C-terminal domain of RNA polymerase II subunit B1 and regulates several aspects of transcription. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 16. [provided by RefSeq, Dec 2014] |
| UniProt Code: | Q96P16 |
| NCBI GenInfo Identifier: | 21361709 |
| NCBI Gene ID: | 55197 |
| NCBI Accession: | NP_060640 |
| UniProt Secondary Accession: | Q96P16,Q7Z5G8, Q96FY9, Q9NVL4, A8KA42, B2RBA3, |
| UniProt Related Accession: | Q96P16 |
| Molecular Weight: | 38.4kDa (337aa) confirmed by MALDI-TOF |
| NCBI Full Name: | regulation of nuclear pre-mRNA domain-containing protein 1A isoform 1 |
| NCBI Synonym Full Names: | regulation of nuclear pre-mRNA domain containing 1A |
| NCBI Official Symbol: | RPRD1A |
| NCBI Official Synonym Symbols: | P15RS; HsT3101 |
| NCBI Protein Information: | regulation of nuclear pre-mRNA domain-containing protein 1A |
| UniProt Protein Name: | Regulation of nuclear pre-mRNA domain-containing protein 1A |
| UniProt Synonym Protein Names: | Cyclin-dependent kinase inhibitor 2B-related protein; p15INK4B-related protein |
| UniProt Gene Name: | RPRD1A |
 | Western blot analysis of extracts of various cell lines, using RPRD1A antibody (CAB15854) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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