Epigenetics & Nuclear Signaling Antibodies 3
Anti-RPP38 Antibody (CAB17080)
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- SKU:
- CAB17080
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-RPP38 Antibody |
| Antibody SKU: | CAB17080 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-110 of human RPP38 (NP_892117.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human |
| Positive Samples: | K-562, HeLa |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-110 of human RPP38 (NP_892117.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | VVKT SLNN PYII RWSA LESE DMHF ILQT LEDR LKAI GLQK IEDK KKKN KTPF LKKE SREK CSIA VDIS ENLK EKKT DAKQ QVSG WTPA HVR |
| Gene ID: | 10557 |
| Uniprot: | P78345 |
| Cellular Location: | |
| Calculated MW: | 31kDa |
| Observed MW: | 38kDa |
| Synonyms: | RPP38 |
| Background: |
| UniProt Protein Function: | RPP38: Component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. RPP38 may associate transiently with RNase P RNA as a factor involved in the transport of H1 RNA to the putative site of its assembly in the cell, the nucleolus. Belongs to the ribosomal protein L7Ae family. |
| UniProt Protein Details: | Protein type:Nucleolus; Ribonuclease; EC 3.1.26.5 Chromosomal Location of Human Ortholog: 10p13 Cellular Component: nucleolar ribonuclease P complex; nucleolus; nucleoplasm; nucleus Molecular Function:protein binding Biological Process: rRNA processing; tRNA 5'-leader removal |
| UniProt Code: | P78345 |
| NCBI GenInfo Identifier: | 83305638 |
| NCBI Gene ID: | 10557 |
| NCBI Accession: | P78345.2 |
| UniProt Secondary Accession: | P78345,Q53F71, Q8NHS8, B3KPY0, D3DRT8, |
| UniProt Related Accession: | P78345 |
| Molecular Weight: | 31,834 Da |
| NCBI Full Name: | Ribonuclease P protein subunit p38 |
| NCBI Synonym Full Names: | ribonuclease P/MRP subunit p38 |
| NCBI Official Symbol: | RPP38 |
| NCBI Protein Information: | ribonuclease P protein subunit p38 |
| UniProt Protein Name: | Ribonuclease P protein subunit p38 |
| Protein Family: | Ribonuclease P protein |
| UniProt Gene Name: | RPP38 |
| UniProt Entry Name: | RPP38_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RPP38 antibody (CAB17080) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded Human lung cancer using RPP38 Rabbit pAb (CAB17080) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U-2 OS cells using RPP38 antibody (CAB17080) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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