Epigenetics & Nuclear Signaling Antibodies 4
Anti-RPP30 Antibody (CAB8479)
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- SKU:
- CAB8479
- Product Type:
- Antibody
- Applications:
- WB
- Applications:
- IHC
- Applications:
- IF
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-RPP30 Antibody |
| Antibody SKU: | CAB8479 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-268 of human RPP30 (NP_006404.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:100 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, Jurkat, HT-1080, HepG2 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-268 of human RPP30 (NP_006404.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAVF ADLD LRAG SDLK ALRG LVET AAHL GYSV VAIN HIVD FKEK KQEI EKPV AVSE LFTT LPIV QGKS RPIK ILTR LTII VSDP SHCN VLRA TSSR ARLY DVVA VFPK TEKL FHIA CTHL DVDL VCIT VTEK LPFY FKRP PINV AIDR GLAF ELVY SPAI KDST MRRY TISS ALNL MQIC KGKN VIIS SAAE RPLE IRGP YDVA NLGL LFGL SESD AKAA VSTN CRAA LLHG ETRK TAFG IIST VKKP RPSE GDED CLPA SKKA KCEG |
| Gene ID: | 10556 |
| Uniprot: | P78346 |
| Cellular Location: | Nucleus, nucleolus |
| Calculated MW: | 29kDa/35kDa |
| Observed MW: | 29kDa |
| Synonyms: | RPP30, TSG15 |
| Background: |
| UniProt Protein Function: | RPP30: Component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. Belongs to the eukaryotic/archaeal RNase P protein component 3 family. |
| UniProt Protein Details: | Protein type:EC 3.1.26.5; Nucleolus; Ribonuclease Chromosomal Location of Human Ortholog: 10q23.31 Cellular Component: nucleolar ribonuclease P complex; nucleoplasm; nucleus Molecular Function:protein binding; ribonuclease P activity; RNA binding Biological Process: rRNA processing; tRNA 5'-leader removal; tRNA processing |
| UniProt Code: | P78346 |
| NCBI GenInfo Identifier: | 13124514 |
| NCBI Gene ID: | 10556 |
| NCBI Accession: | P78346.1 |
| UniProt Secondary Accession: | P78346,B2R799, E9PB02, |
| UniProt Related Accession: | P78346 |
| Molecular Weight: | 29kDa |
| NCBI Full Name: | Ribonuclease P protein subunit p30 |
| NCBI Synonym Full Names: | ribonuclease P/MRP subunit p30 |
| NCBI Official Symbol: | RPP30 |
| NCBI Official Synonym Symbols: | TSG15 |
| NCBI Protein Information: | ribonuclease P protein subunit p30 |
| UniProt Protein Name: | Ribonuclease P protein subunit p30 |
| UniProt Synonym Protein Names: | RNase P subunit 2 |
| Protein Family: | Ribonuclease P protein |
| UniProt Gene Name: | RPP30 |
 | Western blot analysis of extracts of various cell lines, using RPP30 antibody (CAB8479) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human lung cancer using RPP30 antibody (CAB8479) at dilution of 1:100 (40x lens). |
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