Epigenetics & Nuclear Signaling Antibodies 4
Anti-RPP25 Antibody (CAB9973)
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- SKU:
- CAB9973
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-RPP25 Antibody |
| Antibody SKU: | CAB9973 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-199 of human RPP25 (NP_060263.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | A375, HeLa, HT-29 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-199 of human RPP25 (NP_060263.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MENF RKVR SEEA PAGC GAEG GGPG SGPF ADLA PGAV HMRV KEGS KIRN LMAF ATAS MAQP ATRA IVFS GCGR ATTK TVTC AEIL KRRL AGLH QVTR LRYR SVRE VWQS LPPG PTQG QTPG EPAA SLSV LKNV PGLA ILLS KDAL DPRQ PGYQ PPNP HPGP SSPP AAPA SKRS LGEP AAGE GSAK RSQP EPGV ADED QTA |
| Gene ID: | 54913 |
| Uniprot: | Q9BUL9 |
| Cellular Location: | Nucleus |
| Calculated MW: | 20kDa |
| Observed MW: | 20kDa |
| Synonyms: | RPP25 |
| Background: |
| UniProt Protein Function: | RPP25: Component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. Also a component of RNase MRP. This subunit binds to RNA. Belongs to the histone-like Alba family. |
| UniProt Protein Details: | Protein type:Ribonuclease; EC 3.1.26.5 Chromosomal Location of Human Ortholog: 15q24.2 Cellular Component: microtubule organizing center; nucleoplasm Molecular Function:protein binding Biological Process: rRNA processing; tRNA 5'-leader removal |
| UniProt Code: | Q9BUL9 |
| NCBI GenInfo Identifier: | 74733233 |
| NCBI Gene ID: | 54913 |
| NCBI Accession: | Q9BUL9.1 |
| UniProt Secondary Accession: | Q9BUL9,Q9NX88, D3DW70, |
| UniProt Related Accession: | Q9BUL9 |
| Molecular Weight: | 20,632 Da |
| NCBI Full Name: | Ribonuclease P protein subunit p25 |
| NCBI Synonym Full Names: | ribonuclease P/MRP subunit p25 |
| NCBI Official Symbol: | RPP25 |
| NCBI Protein Information: | ribonuclease P protein subunit p25 |
| UniProt Protein Name: | Ribonuclease P protein subunit p25 |
| Protein Family: | Ribonuclease P protein |
| UniProt Gene Name: | RPP25 |
| UniProt Entry Name: | RPP25_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RPP25 antibody (CAB9973) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human colon using RPP25 Rabbit pAb (CAB9973) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse brain using RPP25 Rabbit pAb (CAB9973) at dilution of 1:100 (40x lens). |
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