Antibody Name:	Anti-RPN2 Antibody
Antibody SKU:	CAB8352
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 360-540 of human RPN2 (NP_002942.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, 293T, Jurkat, MCF7, NIH/3T3, Mouse liver, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 360-540 of human RPN2 (NP_002942.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ANTV ELRV KIST EVGI TNVD LSTV DKDQ SIAP KTTR VTYP AKAK GTFI ADSH QNFA LFFQ LVDV NTGA ELTP HQTF VRLH NQKT GQEV VFVA EPDN KNVY KFEL DTSE RKIE FDSA SGTY TLYL IIGD ATLK NPIL WNVA DVVI KFPE EEAP STVL SQNL FTPK QEIQ HLFR EPEK RPPT V
Gene ID:	6185
Uniprot:	P04844
Cellular Location:	Endoplasmic reticulum membrane, Multi-pass membrane protein
Calculated MW:	67kDa/69kDa
Observed MW:	69kDa

Synonyms:

RPN2, RIBIIR, RPN-II, RPNII, SWP1

Background:

This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein is similar in sequence to the yeast oligosaccharyl transferase subunit SWP1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	RPN2: Essential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. Belongs to the SWP1 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 2.4.99.18; Transferase; Membrane protein, multi-pass; Membrane protein, integral; Glycan Metabolism - N-glycan biosynthesis Chromosomal Location of Human Ortholog: 20q12-q13.1 Cellular Component: endoplasmic reticulum membrane; rough endoplasmic reticulum; membrane; endoplasmic reticulum; oligosaccharyl transferase complex; integral to membrane; nucleus Molecular Function:dolichyl-diphosphooligosaccharide-protein glycotransferase activity; ribosome binding Biological Process: response to drug; SRP-dependent cotranslational protein targeting to membrane; cellular protein metabolic process; translation; protein modification process; gene expression; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification; aging
NCBI Summary:	This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein is similar in sequence to the yeast oligosaccharyl transferase subunit SWP1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]
UniProt Code:	P04844
NCBI GenInfo Identifier:	9297108
NCBI Gene ID:	6185
NCBI Accession:	P04844.3
UniProt Secondary Accession:	P04844,Q5JYR6, Q6IBA5, Q96E21, Q9BUQ3, Q9UBE1,
UniProt Related Accession:	P04844
Molecular Weight:	67,723 Da
NCBI Full Name:	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2
NCBI Synonym Full Names:	ribophorin II
NCBI Official Symbol:	RPN2
NCBI Official Synonym Symbols:	SWP1; RPNII; RIBIIR; RPN-II
NCBI Protein Information:	dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2; ribophorin-2; oligosaccharyltransferase complex subunit (non-catalytic); dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa subunit
UniProt Protein Name:	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2
UniProt Synonym Protein Names:	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa subunit; RIBIIR; Ribophorin II; RPN-II; Ribophorin-2
Protein Family:	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase
UniProt Gene Name:	RPN2
UniProt Entry Name:	RPN2_HUMAN

	Western blot analysis of extracts of various cell lines, using RPN2 antibody (CAB8352) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded rat brain using RPN2 antibody (CAB8352) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using RPN2 antibody (CAB8352) at dilution of 1:100 (40x lens).