Antibody Name:	Anti-RPN1 Antibody
Antibody SKU:	CAB6726
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 20-280 of human RPN1 (NP_002941.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Rat
Positive Samples:	HL-60, HepG2, SKOV3, MCF7, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 20-280 of human RPN1 (NP_002941.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	APGS ASSE APPL INED VKRT VDLS SHLA KVTA EVVL AHLG GGST SRAT SFLL ALEP ELEA RLAH LGVQ VKGE DEEE NNLE VRET KIKG KSGR FFTV KLPV ALDP GAKI SVIV ETVY THVL HPYP TQIT QSEK QFVV FEGN HYFY SPYP TKTQ TMRV KLAS RNVE SYTK LGNP TRSE DLLD YGPF RDVP AYSQ DTFK VHYE NNSP FLTI TSMT RVIE VSHW GNIA VEEN VDLK HTGA VLKG PFSR YDYQ RQPD SGIS SIRS F
Gene ID:	6184
Uniprot:	P04843
Cellular Location:	Endoplasmic reticulum membrane, Melanosome, Single-pass type I membrane protein
Calculated MW:	68kDa
Observed MW:	68kDa

Synonyms:

RPN1, OST1, RBPH1

Background:

This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome.

UniProt Protein Function:	RPN1: Essential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. Belongs to the OST1 family.
UniProt Protein Details:	Protein type:EC 2.4.99.18; Glycan Metabolism - N-glycan biosynthesis; Transferase; Membrane protein, integral Chromosomal Location of Human Ortholog: 3q21.3 Cellular Component: endoplasmic reticulum membrane; membrane; rough endoplasmic reticulum; oligosaccharyl transferase complex; integral to membrane; melanosome Molecular Function:protein binding; dolichyl-diphosphooligosaccharide-protein glycotransferase activity Biological Process: SRP-dependent cotranslational protein targeting to membrane; cellular protein metabolic process; translation; protein modification process; protein amino acid N-linked glycosylation via asparagine; gene expression; post-translational protein modification
NCBI Summary:	This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome. [provided by RefSeq, Jul 2008]
UniProt Code:	P04843
NCBI GenInfo Identifier:	132559
NCBI Gene ID:	6184
NCBI Accession:	P04843.1
UniProt Secondary Accession:	P04843,Q68DT1, B2R5Z0, D3DNB6,
UniProt Related Accession:	P04843
Molecular Weight:	607
NCBI Full Name:	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
NCBI Synonym Full Names:	ribophorin I
NCBI Official Symbol:	RPN1
NCBI Official Synonym Symbols:	OST1; RBPH1
NCBI Protein Information:	dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1; RPN-I; ribophorin-1; oligosaccharyltransferase 1 homolog; oligosaccharyltransferase complex subunit (non-catalytic); dolichyl-diphosphooligosaccharide-protein glycosyltransferase 67 kDa subunit; dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit
UniProt Protein Name:	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
UniProt Synonym Protein Names:	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit; Ribophorin I; RPN-I; Ribophorin-1
Protein Family:	26S proteasome regulatory
UniProt Gene Name:	RPN1
UniProt Entry Name:	RPN1_HUMAN

	Western blot analysis of extracts of various cell lines, using RPN1 antibody (CAB6726) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat kidney using RPN1 antibody (CAB6726) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human liver cancer using RPN1 antibody (CAB6726) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human liver damage using RPN1 antibody (CAB6726) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human gastric cancer using RPN1 antibody (CAB6726) at dilution of 1:100 (40x lens).