Antibody Name:	Anti-RPLP0 Antibody
Antibody SKU:	CAB13633
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-317 of human RPLP0 (NP_000993.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	SW620, OVCAR3, Mouse liver, Mouse thymus

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-317 of human RPLP0 (NP_000993.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MPRE DRAT WKSN YFLK IIQL LDDY PKCF IVGA DNVG SKQM QQIR MSLR GKAV VLMG KNTM MRKA IRGH LENN PALE KLLP HIRG NVGF VFTK EDLT EIRD MLLA NKVP AAAR AGAI APCE VTVP AQNT GLGP EKTS FFQA LGIT TKIS RGTI EILS DVQL IKTG DKVG ASEA TLLN MLNI SPFS FGLV IQQV FDNG SIYN PEVL DITE ETLH SRFL EGVR NVAS VCLQ IGYP TVAS VPHS IING YKRV LALS VETD YTFP LAEK VKAF LADP SAFV AAAP VAAA TTAA PAAA AAPA KVEA KEES EESD EDMG FGLF D
Gene ID:	6175
Uniprot:	P05388
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	27kDa/34kDa
Observed MW:	37kDa

Synonyms:

RPLP0, L10E, LP0, P0, PRLP0, RPP0

Background:

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein, which is the functional equivalent of the E. coli L10 ribosomal protein, belongs to the L10P family of ribosomal proteins. It is a neutral phosphoprotein with a C-terminal end that is nearly identical to the C-terminal ends of the acidic ribosomal phosphoproteins P1 and P2. The P0 protein can interact with P1 and P2 to form a pentameric complex consisting of P1 and P2 dimers, and a P0 monomer. The protein is located in the cytoplasm. Transcript variants derived from alternative splicing exist; they encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

UniProt Protein Function:	RPLP0: a ribosomal protein of the L10P family. Located in the cytoplasm and nucleus. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This protein is a component of the 60S subunit.
UniProt Protein Details:	Protein type:Ribosomal; Translation Chromosomal Location of Human Ortholog: 12q24.2 Cellular Component: cytoplasm; cytosol; focal adhesion; membrane; nucleus; ribonucleoprotein complex Molecular Function:protein binding; structural constituent of ribosome Biological Process: mRNA catabolic process, nonsense-mediated decay; rRNA processing; SRP-dependent cotranslational protein targeting to membrane; translational initiation; viral transcription
NCBI Summary:	Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein, which is the functional equivalent of the E. coli L10 ribosomal protein, belongs to the L10P family of ribosomal proteins. It is a neutral phosphoprotein with a C-terminal end that is nearly identical to the C-terminal ends of the acidic ribosomal phosphoproteins P1 and P2. The P0 protein can interact with P1 and P2 to form a pentameric complex consisting of P1 and P2 dimers, and a P0 monomer. The protein is located in the cytoplasm. Transcript variants derived from alternative splicing exist; they encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
UniProt Code:	P05388
NCBI GenInfo Identifier:	133041
NCBI Gene ID:	6175
NCBI Accession:	P05388.1
UniProt Secondary Accession:	P05388,Q3B7A4, Q9BVK4,
UniProt Related Accession:	P05388
Molecular Weight:	27,436 Da
NCBI Full Name:	60S acidic ribosomal protein P0
NCBI Synonym Full Names:	ribosomal protein lateral stalk subunit P0
NCBI Official Symbol:	RPLP0
NCBI Official Synonym Symbols:	P0; LP0; L10E; RPP0; PRLP0
NCBI Protein Information:	60S acidic ribosomal protein P0
UniProt Protein Name:	60S acidic ribosomal protein P0
UniProt Synonym Protein Names:	60S ribosomal protein L10E
UniProt Gene Name:	RPLP0
UniProt Entry Name:	RLA0_HUMAN

	Western blot analysis of extracts of various cell lines, using RPLP0 antibody (CAB13633) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of HeLa cells using RPLP0 antibody (CAB13633). Blue: DAPI for nuclear staining.