Antibody Name:	Anti-RPL9 Antibody
Antibody SKU:	CAB6406
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-192 of human RPL9 (NP_000652.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	U-251MG, HeLa, HepG2, Mouse brain, Mouse pancreas

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-192 of human RPL9 (NP_000652.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MKTI LSNQ TVDI PENV DITL KGRT VIVK GPRG TLRR DFNH INVE LSLL GKKK KRLR VDKW WGNR KELA TVRT ICSH VQNM IKGV TLGF RYKM RSVY AHFP INVV IQEN GSLV EIRN FLGE KYIR RVRM RPGV ACSV SQAQ KDEL ILEG NDIE LVSN SAAL IQQA TTVK NKDI RKFL DGIY VSEK GTVQ QADE
Gene ID:	6133
Uniprot:	P32969
Cellular Location:
Calculated MW:	21kDa
Observed MW:	22kDa

Synonyms:

RPL9, L9, NPC-A-16

Background:

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L6P family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	RPL9: a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L6P family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Protein Details:	Protein type:Translation; Ribosomal Chromosomal Location of Human Ortholog: 4p13 Cellular Component: cytoplasm; cytosol; focal adhesion; membrane; nucleolus; nucleus; ribosome Molecular Function:protein binding; RNA binding Biological Process: mRNA catabolic process, nonsense-mediated decay; rRNA processing; SRP-dependent cotranslational protein targeting to membrane; translational initiation; viral transcription
NCBI Summary:	Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L6P family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]
UniProt Code:	P32969
NCBI GenInfo Identifier:	417677
NCBI Gene ID:	6133
NCBI Accession:	P32969.1
UniProt Related Accession:	P32969
Molecular Weight:	21,863 Da
NCBI Full Name:	60S ribosomal protein L9
NCBI Synonym Full Names:	ribosomal protein L9
NCBI Official Symbol:	RPL9
NCBI Official Synonym Symbols:	L9; NPC-A-16
NCBI Protein Information:	60S ribosomal protein L9
UniProt Protein Name:	60S ribosomal protein L9
UniProt Gene Name:	RPL9
UniProt Entry Name:	RL9_HUMAN

	Western blot analysis of extracts of various cell lines, using RPL9 antibody (CAB6406) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human liver cancer using RPL9 antibody (CAB6406) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of MCF7 cells using RPL9 antibody (CAB6406). Blue: DAPI for nuclear staining.