Antibody Name:	Anti-RPL7A Antibody
Antibody SKU:	CAB14060
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-266 of human RPL7A (NP_000963.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, Jurkat, MCF7, Mouse kidney, Rat kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-266 of human RPL7A (NP_000963.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MPKG KKAK GKKV APAP AVVK KQEA KKVV NPLF EKRP KNFG IGQD IQPK RDLT RFVK WPRY IRLQ RQRA ILYK RLKV PPAI NQFT QALD RQTA TQLL KLAH KYRP ETKQ EKKQ RLLA RAEK KAAG KGDV PTKR PPVL RAGV NTVT TLVE NKKA QLVV IAHD VDPI ELVV FLPA LCRK MGVP YCII KGKA RLGR LVHR KTCT TVAF TQVN SEDK GALA KLVE AIRT NYND RYDE IRRH WGGN VLGP KSVA RIAK LEKA KAKE LATK LG
Gene ID:	6130
Uniprot:	P62424
Cellular Location:
Calculated MW:	29kDa
Observed MW:	35kDa

Synonyms:

RPL7A, L7A, SURF3, TRUP

Background:

Cytoplasmic ribosomes, organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L7AE family of ribosomal proteins. It can interact with a subclass of nuclear hormone receptors, including thyroid hormone receptor, and inhibit their ability to transactivate by preventing their binding to their DNA response elements. This gene is included in the surfeit gene cluster, a group of very tightly linked genes that do not share sequence similarity. It is co-transcribed with the U24, U36a, U36b, and U36c small nucleolar RNA genes, which are located in its second, fifth, fourth, and sixth introns, respectively. This gene rearranges with the trk proto-oncogene to form the chimeric oncogene trk-2h, which encodes an oncoprotein consisting of the N terminus of ribosomal protein L7a fused to the receptor tyrosine kinase domain of trk. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

UniProt Protein Function:	RPL7A: Belongs to the ribosomal protein L7Ae family.
UniProt Protein Details:	Protein type:Nuclear receptor co-regulator; Translation; Ribosomal Chromosomal Location of Human Ortholog: 9q34 Cellular Component: cytoplasm; cytosol; focal adhesion; membrane; nucleolus; nucleus; polysomal ribosome Molecular Function:protein binding; RNA binding Biological Process: maturation of LSU-rRNA; mRNA catabolic process, nonsense-mediated decay; rRNA processing; SRP-dependent cotranslational protein targeting to membrane; translational initiation; viral transcription
NCBI Summary:	Cytoplasmic ribosomes, organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L7AE family of ribosomal proteins. It can interact with a subclass of nuclear hormone receptors, including thyroid hormone receptor, and inhibit their ability to transactivate by preventing their binding to their DNA response elements. This gene is included in the surfeit gene cluster, a group of very tightly linked genes that do not share sequence similarity. It is co-transcribed with the U24, U36a, U36b, and U36c small nucleolar RNA genes, which are located in its second, fifth, fourth, and sixth introns, respectively. This gene rearranges with the trk proto-oncogene to form the chimeric oncogene trk-2h, which encodes an oncoprotein consisting of the N terminus of ribosomal protein L7a fused to the receptor tyrosine kinase domain of trk. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
UniProt Code:	P62424
NCBI GenInfo Identifier:	54039239
NCBI Gene ID:	6130
NCBI Accession:	P62424.2
UniProt Secondary Accession:	P62424,P11518, Q5T8U4,
UniProt Related Accession:	P62424
Molecular Weight:	29,996 Da
NCBI Full Name:	60S ribosomal protein L7a
NCBI Synonym Full Names:	ribosomal protein L7a
NCBI Official Symbol:	RPL7A
NCBI Official Synonym Symbols:	L7A; TRUP; SURF3
NCBI Protein Information:	60S ribosomal protein L7a
UniProt Protein Name:	60S ribosomal protein L7a
UniProt Synonym Protein Names:	PLA-X polypeptide; Surfeit locus protein 3
Protein Family:	60S ribosomal protein
UniProt Gene Name:	RPL7A
UniProt Entry Name:	RL7A_HUMAN

	Western blot analysis of extracts of various cell lines, using RPL7A antibody (CAB14060) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunofluorescence analysis of C6 cells using RPL7A antibody (CAB14060) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using RPL7A antibody (CAB14060) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using RPL7A antibody (CAB14060) at dilution of 1:100. Blue: DAPI for nuclear staining.