Antibody Name:	Anti-RPL7 Antibody
Antibody SKU:	CAB5932
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-248 of human RPL7 (NP_000962.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, MCF7, A375, HeLa, 293T, Mouse kidney, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-248 of human RPL7 (NP_000962.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MEGV EEKK KEVP AVPE TLKK KRRN FAEL KIKR LRKK FAQK MLRK ARRK LIYE KAKH YHKE YRQM YRTE IRMA RMAR KAGN FYVP AEPK LAFV IRIR GING VSPK VRKV LQLL RLRQ IFNG TFVK LNKA SINM LRIV EPYI AWGY PNLK SVNE LIYK RGYG KINK KRIA LTDN ALIA RSLG KYGI ICME DLIH EIYT VGKR FKEA NNFL WPFK LSSP RGGM KKKT THFV EGGD AGNR EDQI NRLI RRMN
Gene ID:	6129
Uniprot:	P18124
Cellular Location:
Calculated MW:	29kDa
Observed MW:	29kDa

Synonyms:

RPL7, L7, humL7-1

Background:

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L30P family of ribosomal proteins. It contains an N-terminal basic region-leucine zipper (BZIP)-like domain and the RNP consensus submotif RNP2. In vitro the BZIP-like domain mediates homodimerization and stable binding to DNA and RNA, with a preference for 28S rRNA and mRNA. The protein can inhibit cell-free translation of mRNAs, suggesting that it plays a regulatory role in the translation apparatus. It is located in the cytoplasm. The protein has been shown to be an autoantigen in patients with systemic autoimmune diseases, such as systemic lupus erythematosus. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

UniProt Protein Function:	RPL7: a ribosomal protein of the L30P family. Located in the cytoplasm. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This protein is a component of the 60S subunit. Contains an N-terminal basic region-leucine zipper (BZIP)-like domain and the RNP consensus submotif RNP2. In vitro the BZIP-like domain mediates homodimerization and stable binding to DNA and RNA, with a preference for 28S rRNA and mRNA. Can inhibit cell-free translation of mRNAs, suggesting that it plays a regulatory role in the translation apparatus.
UniProt Protein Details:	Protein type:RNA processing; Nuclear receptor co-regulator; Ribosomal Chromosomal Location of Human Ortholog: 8q21.11 Cellular Component: cytoplasm; cytosol; focal adhesion; membrane; nucleolus; nucleus; ribonucleoprotein complex Molecular Function:DNA binding; mRNA binding; protein binding; protein homodimerization activity; RNA binding Biological Process: maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); mRNA catabolic process, nonsense-mediated decay; ribosomal large subunit biogenesis and assembly; rRNA processing; SRP-dependent cotranslational protein targeting to membrane; translational initiation; viral transcription
NCBI Summary:	Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L30P family of ribosomal proteins. It contains an N-terminal basic region-leucine zipper (BZIP)-like domain and the RNP consensus submotif RNP2. In vitro the BZIP-like domain mediates homodimerization and stable binding to DNA and RNA, with a preference for 28S rRNA and mRNA. The protein can inhibit cell-free translation of mRNAs, suggesting that it plays a regulatory role in the translation apparatus. It is located in the cytoplasm. The protein has been shown to be an autoantigen in patients with systemic autoimmune diseases, such as systemic lupus erythematosus. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
UniProt Code:	P18124
NCBI GenInfo Identifier:	133021
NCBI Gene ID:	6129
NCBI Accession:	P18124.1
UniProt Secondary Accession:	P18124,Q15289, Q3KQU0, Q5I0X1, Q6IBM9, A8K504,
UniProt Related Accession:	P18124
Molecular Weight:	29,226 Da
NCBI Full Name:	60S ribosomal protein L7
NCBI Synonym Full Names:	ribosomal protein L7
NCBI Official Symbol:	RPL7
NCBI Official Synonym Symbols:	L7; humL7-1
NCBI Protein Information:	60S ribosomal protein L7
UniProt Protein Name:	60S ribosomal protein L7
Protein Family:	60S ribosomal protein
UniProt Gene Name:	RPL7
UniProt Entry Name:	RL7_HUMAN

	Western blot analysis of extracts of various cell lines, using RPL7 antibody (CAB5932) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded rat brain using RPL7 antibody (CAB5932) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophageal using RPL7 antibody (CAB5932) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using RPL7 antibody (CAB5932) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of L929 cells using RPL7 Polyclonal Antibody (CAB5932) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.