Antibody Name:	Anti-RPL36AL Antibody
Antibody SKU:	CAB14366
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-106 of human RPL36AL (NP_000992.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, A-549, HeLa, Jurkat, Mouse kidney, Mouse lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-106 of human RPL36AL (NP_000992.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MVNV PKTR RTFC KKCG KHQP HKVT QYKK GKDS LYAQ GRRR YDRK QSGY GGQT KPIF RKKA KTTK KIVL RLEC VEPN CRSK RMLA IKRC KHFE LGGD KKRK GQVI QF
Gene ID:	6166
Uniprot:	Q969Q0
Cellular Location:	Cytoplasm
Calculated MW:	12kDa
Observed MW:	16kDa

Synonyms:

RPL36AL, RPL36A

Background:

Cytoplasmic ribosomes, organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein, which shares sequence similarity with yeast ribosomal protein L44, belongs to the L44E (L36AE) family of ribosomal proteins. This gene and the human gene officially named ribosomal protein L36a (RPL36A) encode nearly identical proteins; however, they are distinct genes. Although the name of this gene has been referred to as ribosomal protein L36a (RPL36A), its official name is ribosomal protein L36a-like (RPL36AL). As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

UniProt Protein Function:	RPL36AL: Cytoplasmic ribosomes, organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein, which shares sequence similarity with yeast ribosomal protein L44, belongs to the L44E (L36AE) family of ribosomal proteins. This gene and the human gene officially named ribosomal protein L36a (RPL36A) encode nearly identical proteins; however, they are distinct genes. Although the name of this gene has been referred to as ribosomal protein L36a (RPL36A), its official name is ribosomal protein L36a-like (RPL36AL). As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 14q21.3 Cellular Component: cytosol; nucleus; plasma membrane Molecular Function:protein binding
NCBI Summary:	Cytoplasmic ribosomes, organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein, which shares sequence similarity with yeast ribosomal protein L44, belongs to the L44E (L36AE) family of ribosomal proteins. This gene and the human gene officially named ribosomal protein L36a (RPL36A) encode nearly identical proteins; however, they are distinct genes. Although the name of this gene has been referred to as ribosomal protein L36a (RPL36A), its official name is ribosomal protein L36a-like (RPL36AL). As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
UniProt Code:	Q969Q0
NCBI GenInfo Identifier:	4506651
NCBI Gene ID:	6166
NCBI Accession:	NP_000992.1
UniProt Secondary Accession:	Q969Q0,Q3B7A5,
UniProt Related Accession:	Q969Q0
Molecular Weight:	39.7kD
NCBI Full Name:	60S ribosomal protein L36a-like
NCBI Synonym Full Names:	ribosomal protein L36a like
NCBI Official Symbol:	RPL36AL
NCBI Official Synonym Symbols:	RPL36A
NCBI Protein Information:	60S ribosomal protein L36a-like
UniProt Protein Name:	60S ribosomal protein L36a-like
UniProt Synonym Protein Names:	Large ribosomal subunit protein eL42-like
Protein Family:	60S ribosomal protein
UniProt Gene Name:	RPL36AL

	Western blot analysis of extracts of various cell lines, using RPL36AL antibody (CAB14366) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of C6 cells using RPL36AL Polyclonal Antibody (CAB14366) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using RPL36AL Polyclonal Antibody (CAB14366) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using RPL36AL Polyclonal Antibody (CAB14366) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Confocal immunofluorescence analysis of HeLa cells using RPL36AL Polyclonal Antibody (CAB14366) at dilution of 1:100. Blue: DAPI for nuclear staining.