Cell Biology Antibodies 12

Anti-RPL22 Antibody (CAB9202)

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SKU:
CAB9202
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-RPL22 Antibody
Antibody SKU:CAB9202
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-122 of human RPL22 (NP_000974.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, U-87MG, HepG2, Jurkat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-122 of human RPL22 (NP_000974.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAPV KKLV VKGG KKKK QVLK FTLD CTHP VEDG IMDA ANFE QFLQ ERIK VNGK AGNL GGGV VTIE RSKS KITV TSEV PFSK RYLK YLTK KYLK KNNL RDWL RVVA NSKE SYEL RYFQ INQD EE
Gene ID:6146
Uniprot:P35268
Cellular Location:
Calculated MW:14kDa
Observed MW:15kDa
Synonyms:RPL22, EAP, HBP15, HBP15/L22, L22
Background:Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 60S subunit. The protein belongs to the L22E family of ribosomal proteins. Its initiating methionine residue is post-translationally removed. The protein can bind specifically to Epstein-Barr virus-encoded RNAs (EBERs) 1 and 2. The mouse protein has been shown to be capable of binding to heparin. Transcript variants utilizing alternative polyA signals exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. It was previously thought that this gene mapped to 3q26 and that it was fused to the acute myeloid leukemia 1 (AML1) gene located at 21q22 in some therapy-related myelodysplastic syndrome patients with 3;21 translocations; however, these fusions actually involve a ribosomal protein L22 pseudogene located at 3q26, and this gene actually maps to 1p36.3-p36.2.
UniProt Protein Function:RPL22: a cytoplasmic ribosomal protein that is a component of the 60S subunit. The protein belongs to the L22E family of ribosomal proteins. Its initiating methionine residue is post-translationally removed. The protein can bind specifically to Epstein-Barr virus-encoded RNAs (EBERs) 1 and 2. The mouse protein has been shown to be capable of binding to heparin. Transcript variants utilizing alternative polyA signals exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. It was previously thought that this gene mapped to 3q26 and that it was fused to the acute myeloid leukemia 1 (AML1) gene located at 21q22 in some therapy-related myelodysplastic syndrome patients with 3;21 translocations; however, these fusions actually involve a ribosomal protein L22 pseudogene located at 3q26, and this gene actually maps to 1p36.3-p36.2. [provided by RefSeq, Jul 2008]
UniProt Protein Details:

Protein type:RNA-binding

Chromosomal Location of Human Ortholog: 1p36.31

Cellular Component: focal adhesion; cytoplasm; ribonucleoprotein complex; nucleus; cytosol

Molecular Function:heparin binding; protein binding; structural constituent of ribosome; RNA binding

Biological Process: SRP-dependent cotranslational protein targeting to membrane; cellular protein metabolic process; translational elongation; translation; viral reproduction; mRNA catabolic process, nonsense-mediated decay; translational initiation; alpha-beta T cell differentiation; gene expression; viral transcription; translational termination; viral infectious cycle

NCBI Summary:Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 60S subunit. The protein belongs to the L22E family of ribosomal proteins. Its initiating methionine residue is post-translationally removed. The protein can bind specifically to Epstein-Barr virus-encoded RNAs (EBERs) 1 and 2. The mouse protein has been shown to be capable of binding to heparin. Transcript variants utilizing alternative polyA signals exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. It was previously thought that this gene mapped to 3q26 and that it was fused to the acute myeloid leukemia 1 (AML1) gene located at 21q22 in some therapy-related myelodysplastic syndrome patients with 3;21 translocations; however, these fusions actually involve a ribosomal protein L22 pseudogene located at 3q26, and this gene actually maps to 1p36.3-p36.2. [provided by RefSeq, Jul 2008]
UniProt Code:P35268
NCBI GenInfo Identifier:4506613
NCBI Gene ID:6146
NCBI Accession:NP_000974.1
UniProt Secondary Accession:P35268,Q6IBD1, B2R495,
UniProt Related Accession:P35268
Molecular Weight:
NCBI Full Name:60S ribosomal protein L22 proprotein
NCBI Synonym Full Names:ribosomal protein L22
NCBI Official Symbol:RPL22  
NCBI Official Synonym Symbols:EAP; L22; HBP15; HBP15/L22  
NCBI Protein Information:60S ribosomal protein L22
UniProt Protein Name:60S ribosomal protein L22
UniProt Synonym Protein Names:EBER-associated protein; EAP; Epstein-Barr virus small RNA-associated protein; Heparin-binding protein HBp15
Protein Family:50S ribosomal protein
UniProt Gene Name:RPL22  
UniProt Entry Name:RL22_HUMAN
Anti-RPL22 Polyclonal Antibody (CAB9202)Western blot analysis of extracts of various cell lines, using RPL22 antibody (CAB9202) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-RPL22 Polyclonal Antibody (CAB9202)Immunohistochemistry of paraffin-embedded rat lung using RPL22 antibody (CAB9202) at dilution of 1:100 (40x lens).
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