Cell Biology Antibodies 2

Anti-RPL18 Antibody (CAB10720)

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SKU:
CAB10720
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-RPL18 Antibody
Antibody SKU:CAB10720
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-188 of human RPL18 (NP_000970.1).
Application:WB
Recommended Dilution:WB 1:1000 - 1:3000
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, HT-29, A-549, HeLa, HepG2, Mouse brain, Mouse heart, Mouse kidney, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-188 of human RPL18 (NP_000970.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MGVD IRHN KDRK VRRK EPKS QDIY LRLL VKLY RFLA RRTN STFN QVVL KRLF MSRT NRPP LSLS RMIR KMKL PGRE NKTA VVVG TITD DVRV QEVP KLKV CALR VTSR ARSR ILRA GGKI LTFD QLAL DSPK GCGT VLLS GPRK GREV YRHF GKAP GTPH SHTK PYVR SKGR KFER ARGR RASR GYKN
Gene ID:6141
Uniprot:Q07020
Cellular Location:Cytoplasm
Calculated MW:18kDa/21kDa
Observed MW:22kDa
Synonyms:RPL18, L18
Background:Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a member of the L18E family of ribosomal proteins that is a component of the 60S subunit. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
UniProt Protein Function:RPL18: a member of the L18E family of ribosomal proteins that is a component of the 60S subunit. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]
UniProt Protein Details:

Protein type:Ribosomal; Translation

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: cytosol; endoplasmic reticulum; focal adhesion; membrane; nucleolus; nucleus

Molecular Function:RNA binding

Biological Process: mRNA catabolic process, nonsense-mediated decay; rRNA processing; SRP-dependent cotranslational protein targeting to membrane; translational initiation; viral transcription

NCBI Summary:Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a member of the L18E family of ribosomal proteins that is a component of the 60S subunit. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]
UniProt Code:Q07020
NCBI GenInfo Identifier:548749
NCBI Gene ID:6141
NCBI Accession:Q07020.2
UniProt Secondary Accession:Q07020,Q8WTZ6, F8VWC5,
UniProt Related Accession:Q07020
Molecular Weight:22kDa
NCBI Full Name:60S ribosomal protein L18
NCBI Synonym Full Names:ribosomal protein L18
NCBI Official Symbol:RPL18  
NCBI Official Synonym Symbols:L18  
NCBI Protein Information:60S ribosomal protein L18
UniProt Protein Name:60S ribosomal protein L18
UniProt Synonym Protein Names:Large ribosomal subunit protein eL18
Protein Family:Ribosomal protein
UniProt Gene Name:RPL18  
Anti-RPL18 Antibody (CAB10720)Western blot analysis of extracts of various cell lines, using RPL18 antibody (CAB10720) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
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