Antibody Name:	Anti-RPL17 Antibody
Antibody SKU:	CAB5934
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-184 of human RPL17 (NP_000976.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:200 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, 293T, A-549, SW480, HeLa, Mouse lung, Mouse thymus, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-184 of human RPL17 (NP_000976.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MVRY SLDP ENPT KSCK SRGS NLRV HFKN TRET AQAI KGMH IRKA TKYL KDVT LQKQ CVPF RRYN GGVG RCAQ AKQW GWTQ GRWP KKSA EFLL HMLK NAES NAEL KGLD VDSL VIEH IQVN KAPK MRRR TYRA HGRI NPYM SSPC HIEM ILTE KEQI VPKP EEEV AQKK KISQ KKLK KQKL MARE
Gene ID:	6139
Uniprot:	P18621
Cellular Location:
Calculated MW:	17kDa/21kDa/26kDa
Observed MW:	21kDa

Synonyms:

RPL17, L17, PD-1, RPL23

Background:

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L22P family of ribosomal proteins. It is located in the cytoplasm. This gene has been referred to as rpL23 because the encoded protein shares amino acid identity with ribosomal protein L23 from Halobacterium marismortui; however, its official symbol is RPL17. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream C18orf32 (chromosome 18 open reading frame 32) gene.

UniProt Protein Function:	RPL17: a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L22P family of ribosomal proteins. It is located in the cytoplasm. This gene has been referred to as rpL23 because the encoded protein shares amino acid identity with ribosomal protein L23 from Halobacterium marismortui; however, its official symbol is RPL17. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream C18orf32 (chromosome 18 open reading frame 32) gene. [provided by RefSeq, Dec 2010]
UniProt Protein Details:	Protein type:Translation; Ribosomal Chromosomal Location of Human Ortholog: 18q21 Cellular Component: nucleus; cytosol Molecular Function:structural constituent of ribosome Biological Process: SRP-dependent cotranslational protein targeting to membrane; translational elongation; cellular protein metabolic process; viral reproduction; translation; mRNA catabolic process, nonsense-mediated decay; translational initiation; gene expression; viral transcription; viral infectious cycle; translational termination
NCBI Summary:	This locus represents naturally occurring read-through transcription between the neighboring RPL17 (ribosomal protein L17) and C18orf32 (chromosome 18 open reading frame 32) genes. Alternative splicing results in multiple transcript variants. The encoded isoforms share sequence identity with the RPL17 protein, but they include frameshifted C-terminal regions derived from the downstream gene exons. [provided by RefSeq, Dec 2010]
UniProt Code:	P18621
NCBI GenInfo Identifier:	132799
NCBI Gene ID:	100526842
NCBI Accession:	P18621.3
UniProt Secondary Accession:	P18621,Q3KQW2, Q6NZ54, Q7M4M5, B2R4H3, B4E3C2, B5ME31 J3QL51,
Molecular Weight:	184
NCBI Full Name:	60S ribosomal protein L17
NCBI Synonym Full Names:	RPL17-C18orf32 readthrough
NCBI Official Symbol:	RPL17-C18orf32
NCBI Protein Information:	RPL17-C18orf32 protein
UniProt Protein Name:	60S ribosomal protein L17
UniProt Synonym Protein Names:	60S ribosomal protein L23; PD-1
Protein Family:	60S ribosomal protein
UniProt Gene Name:	RPL17
UniProt Entry Name:	RL17_HUMAN

	Western blot analysis of extracts of various cell lines, using RPL17 antibody (CAB5934) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded rat brain using RPL17 Rabbit pAb (CAB5934) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human placenta using RPL17 Rabbit pAb (CAB5934) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using RPL17 Rabbit pAb (CAB5934) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using RPL17 antibody (CAB5934) at dilution of 1:100. Blue: DAPI for nuclear staining.