Antibody Name:	Anti-RPL10A Antibody
Antibody SKU:	CAB5925
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human RPL10A (NP_009035.3).

Application:	WB IHC
Recommended Dilution:	WB 1:1000 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, HepG2, HT-1080, NIH/3T3, HL-60, Mouse lung, Mouse kidney, Mouse pancreas, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human RPL10A (NP_009035.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSSK VSRD TLYE AVRE VLHG NQRK RRKF LETV ELQI SLKN YDPQ KDKR FSGT VRLK STPR PKFS VCVL GDQQ HCDE AKAV DIPH MDIE ALKK LNKN KKLV KKLA KKYD AF
Gene ID:	4736
Uniprot:	P62906
Cellular Location:
Calculated MW:	24kDa
Observed MW:	25kDa

Synonyms:

RPL10A, CSA19, Csa-19, L10A, NEDD6

Background:

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L1P family of ribosomal proteins. It is located in the cytoplasm. The expression of this gene is downregulated in the thymus by cyclosporin-A (CsA), an immunosuppressive drug. Studies in mice have shown that the expression of the ribosomal protein L10a gene is downregulated in neural precursor cells during development. This gene previously was referred to as NEDD6 (neural precursor cell expressed, developmentally downregulated 6), but it has been renamed RPL10A (ribosomal protein 10a). As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

UniProt Protein Function:	RPL10A: a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L1P family of ribosomal proteins. It is located in the cytoplasm. The expression of this gene is downregulated in the thymus by cyclosporin-A (CsA), an immunosuppressive drug. Studies in mice have shown that the expression of the ribosomal protein L10a gene is downregulated in neural precursor cells during development. This gene previously was referred to as NEDD6 (neural precursor cell expressed, developmentally downregulated 6), but it has been renamed RPL10A (ribosomal protein 10a). As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
UniProt Protein Details:	Protein type:Ribosomal; Translation Chromosomal Location of Human Ortholog: 6p21.31 Cellular Component: focal adhesion; membrane; mitochondrion; cytoplasm; nucleolus; cytosol; nucleus Molecular Function:protein binding; structural constituent of ribosome Biological Process: SRP-dependent cotranslational protein targeting to membrane; anatomical structure morphogenesis; translational elongation; cellular protein metabolic process; viral reproduction; translation; translational initiation; mRNA catabolic process, nonsense-mediated decay; gene expression; viral transcription; viral infectious cycle; translational termination
NCBI Summary:	Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L1P family of ribosomal proteins. It is located in the cytoplasm. The expression of this gene is downregulated in the thymus by cyclosporin-A (CsA), an immunosuppressive drug. Studies in mice have shown that the expression of the ribosomal protein L10a gene is downregulated in neural precursor cells during development. This gene previously was referred to as NEDD6 (neural precursor cell expressed, developmentally downregulated 6), but it has been renamed RPL10A (ribosomal protein 10a). As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
UniProt Code:	P62906
NCBI GenInfo Identifier:	51702773
NCBI Gene ID:	4736
NCBI Accession:	P62906.2
UniProt Secondary Accession:	P62906,P52859, P53025, Q5TZT6, Q8J013, B2R801,
UniProt Related Accession:	P62906
Molecular Weight:
NCBI Full Name:	60S ribosomal protein L10a
NCBI Synonym Full Names:	ribosomal protein L10a
NCBI Official Symbol:	RPL10A
NCBI Official Synonym Symbols:	L10A; CSA19; NEDD6; Csa-19
NCBI Protein Information:	60S ribosomal protein L10a; NEDD-6; neural precursor cell expressed developmentally down-regulated protein 6
UniProt Protein Name:	60S ribosomal protein L10a
UniProt Synonym Protein Names:	CSA-19; Neural precursor cell expressed developmentally down-regulated protein 6; NEDD-6
Protein Family:	60S ribosomal protein
UniProt Gene Name:	RPL10A
UniProt Entry Name:	RL10A_HUMAN

	Western blot analysis of extracts of various cell lines, using RPL10A antibody (CAB5925) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded rat cerebellum using RPL10A Rabbit pAb (CAB5925) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using RPL10A Rabbit pAb (CAB5925) at dilution of 1:100 (40x lens).