Cell Biology Antibodies 10

Anti-RPH3A Antibody (CAB6722)

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SKU:
CAB6722
Product Type:
Antibody
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-RPH3A Antibody
Antibody SKU:CAB6722
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human RPH3A (NP_055769.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse, Rat
Positive Samples:Mouse brain, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human RPH3A (NP_055769.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MTDT VFSN SSNR WMYP SDRP LQSK LQAG WSVH PGGQ PDRQ RKQE ELTD EEKE IINR VIAR AEKM EEME QERI GRLV DRLE NMRK NVAG DGVN RCIL CGEQ LGML GSAC VVCE DCKK NVCT KCGV ETNN RLHS VWLC KICI EQRE VWKR SGAW FFKG FPKQ VLPQ PMPI KKTK PQQP VSEP AAPE QPAP EPKH PARA PARG DSED RRGP GQKT GPDP ASAP GRGN YGPP VRRA SEAR MSSS SRDS ESWD HSGG AGDS SRSP AGLR RANS VQAS RPAP GSVQ
Gene ID:22895
Uniprot:Q9Y2J0
Cellular Location:Cell junction, Membrane, Peripheral membrane protein, synapse
Calculated MW:76kDa
Observed MW:95kDa
Synonyms:RPH3A
Background:The protein encoded by this gene is thought to be an effector for RAB3A, which is a small G protein that acts in the late stages of neurotransmitter exocytosis. The encoded protein may be involved in neurotransmitter release and synaptic vesicle traffic.
UniProt Protein Function:rabphilin 3A: Protein transport. Probably involved with Ras-related protein Rab-3A in synaptic vesicle traffic and/or synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Vesicle

Chromosomal Location of Human Ortholog: 12q24.13

Cellular Component: Golgi apparatus; synaptic vesicle; neuron projection; protein complex; extrinsic to membrane; synaptic vesicle membrane; synapse; cell junction; cytosol; secretory granule

Molecular Function:phosphatidylinositol-4,5-bisphosphate binding; selenium binding; protein binding; zinc ion binding; calcium-dependent phospholipid binding; transporter activity; protein complex binding; calcium ion binding; phosphate binding; Rab GTPase binding

Biological Process: intracellular protein transport

NCBI Summary:Exocytosis of neurotransmitters and hormones is fundamental to synaptic neurotransmission and cell-cell communication. RAB3A (MIM 179390) is a small G protein that is thought to act at late stages of exocytosis, and RPH3A is a RAB3A effector (Lin et al., 2007 [PubMed 17149709]).[supplied by OMIM, Jul 2008]
UniProt Code:Q9Y2J0
NCBI GenInfo Identifier:13878745
NCBI Gene ID:22895
NCBI Accession:Q9Y2J0.1
UniProt Secondary Accession:Q9Y2J0,Q96AE0, B7Z3C3,
UniProt Related Accession:Q9Y2J0
Molecular Weight:694
NCBI Full Name:Rabphilin-3A
NCBI Synonym Full Names:rabphilin 3A
NCBI Official Symbol:RPH3A  
NCBI Protein Information:rabphilin-3A; exophilin-1; rabphilin 3A homolog
UniProt Protein Name:Rabphilin-3A
UniProt Synonym Protein Names:Exophilin-1
Protein Family:Rabphilin
UniProt Gene Name:RPH3A  
UniProt Entry Name:RP3A_HUMAN
Anti-RPH3A Antibody (CAB6722)Western blot analysis of extracts of various cell lines, using RPH3A antibody (CAB6722) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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