Cell Biology Antibodies 4
Anti-RPH3A Antibody (CAB13584)
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- SKU:
- CAB13584
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-RPH3A Antibody |
| Antibody SKU: | CAB13584 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human RPH3A (NP_055769.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse brain, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human RPH3A (NP_055769.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MTDT VFSN SSNR WMYP SDRP LQSK LQAG WSVH PGGQ PDRQ RKQE ELTD EEKE IINR VIAR AEKM EEME QERI GRLV DRLE NMRK NVAG DGVN RCIL CGEQ LGML GSAC VVCE DCKK NVCT KCGV ETNN RLHS VWLC KICI EQRE VWKR SGAW FFKG FPKQ VLPQ PMPI KKTK PQQP VSEP AAPE QPAP EPKH PARA PARG DSED RRGP GQKT GPDP ASAP GRGN YGPP VRRA SEAR MSSS SRDS ESWD HSGG AGDS SRSP AGLR RANS VQAS RPAP GSVQ |
| Gene ID: | 22895 |
| Uniprot: | Q9Y2J0 |
| Cellular Location: | Cell junction, Membrane, Peripheral membrane protein, synapse |
| Calculated MW: | 76kDa |
| Observed MW: | 77kDa |
| Synonyms: | RPH3A |
| Background: | The protein encoded by this gene is thought to be an effector for RAB3A, which is a small G protein that acts in the late stages of neurotransmitter exocytosis. The encoded protein may be involved in neurotransmitter release and synaptic vesicle traffic. |
| UniProt Protein Function: | rabphilin 3A: Protein transport. Probably involved with Ras-related protein Rab-3A in synaptic vesicle traffic and/or synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Vesicle Chromosomal Location of Human Ortholog: 12q24.13 Cellular Component: Golgi apparatus; synaptic vesicle; neuron projection; protein complex; extrinsic to membrane; synaptic vesicle membrane; synapse; cell junction; cytosol; secretory granule Molecular Function:phosphatidylinositol-4,5-bisphosphate binding; selenium binding; protein binding; zinc ion binding; calcium-dependent phospholipid binding; transporter activity; protein complex binding; calcium ion binding; phosphate binding; Rab GTPase binding Biological Process: intracellular protein transport |
| NCBI Summary: | Exocytosis of neurotransmitters and hormones is fundamental to synaptic neurotransmission and cell-cell communication. RAB3A (MIM 179390) is a small G protein that is thought to act at late stages of exocytosis, and RPH3A is a RAB3A effector (Lin et al., 2007 [PubMed 17149709]).[supplied by OMIM, Jul 2008] |
| UniProt Code: | Q9Y2J0 |
| NCBI GenInfo Identifier: | 13878745 |
| NCBI Gene ID: | 22895 |
| NCBI Accession: | Q9Y2J0.1 |
| UniProt Secondary Accession: | Q9Y2J0,Q96AE0, B7Z3C3, |
| UniProt Related Accession: | Q9Y2J0 |
| Molecular Weight: | 694 |
| NCBI Full Name: | Rabphilin-3A |
| NCBI Synonym Full Names: | rabphilin 3A |
| NCBI Official Symbol: | RPH3A |
| NCBI Protein Information: | rabphilin-3A; exophilin-1; rabphilin 3A homolog |
| UniProt Protein Name: | Rabphilin-3A |
| UniProt Synonym Protein Names: | Exophilin-1 |
| Protein Family: | Rabphilin |
| UniProt Gene Name: | RPH3A |
| UniProt Entry Name: | RP3A_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RPH3A antibody (CAB13584) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded human liver cancer using RPH3A antibody (CAB13584) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human colon carcinoma using RPH3A antibody (CAB13584) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse heart using RPH3A antibody (CAB13584) at dilution of 1:100 (40x lens). |
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