Metabolism Antibodies 1
Anti-RPE Antibody (CAB14778)
- SKU:
- CAB14778
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-RPE Antibody |
| Antibody SKU: | CAB14778 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-228 of human RPE (NP_954699.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | K-562, HeLa, 293T, Mouse brain, Mouse heart, Mouse thymus, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-228 of human RPE (NP_954699.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MASG CKIG PSIL NSDL ANLG AECL RMLD SGAD YLHL DVMD GHFV PNIT FGHP VVES LRKQ LGQD PFFD MHMM VSKP EQWV KPMA VAGA NQYT FHLE ATEN PGAL IKDI RENG MKVG LAIK PGTS VEYL APWA NQID MALV MTVE PGFG GQKF MEDM MPKV HWLR TQFP SLDI EVDG GVGP DTVH KCAE AGAN MIVS GSAI MRSE DPRS VINL LRNV CSEA AQKR SLDR |
| Gene ID: | 6120 |
| Uniprot: | Q96AT9 |
| Cellular Location: | |
| Calculated MW: | 17kDa/19kDa/21kDa/23kDa/24kDa |
| Observed MW: | 25kDa |
| Synonyms: | RPE, RPE2-1 |
| Background: |
| UniProt Protein Function: | RPE: Catalyzes the reversible epimerization of D-ribulose 5- phosphate to D-xylulose 5-phosphate. Belongs to the ribulose-phosphate 3-epimerase family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Isomerase; Carbohydrate Metabolism - pentose and glucuronate interconversions; EC 5.1.3.1; Carbohydrate Metabolism - pentose phosphate pathway Chromosomal Location of Human Ortholog: 2q32-q33.3 Cellular Component: cytosol Molecular Function:identical protein binding; protein homodimerization activity; ribulose-phosphate 3-epimerase activity; metal ion binding Biological Process: pentose-phosphate shunt; carbohydrate metabolic process; pentose-phosphate shunt, non-oxidative branch; pentose catabolic process; pathogenesis; cellular carbohydrate metabolic process |
| UniProt Code: | Q96AT9 |
| NCBI GenInfo Identifier: | 506949360 |
| NCBI Gene ID: | 6120 |
| NCBI Accession: | NP_001265211.1 |
| UniProt Secondary Accession: | Q96AT9,Q8VEE0, |
| UniProt Related Accession: | Q96AT9 |
| Molecular Weight: | 17,570 Da |
| NCBI Full Name: | ribulose-phosphate 3-epimerase isoform 2 |
| NCBI Synonym Full Names: | ribulose-5-phosphate-3-epimerase |
| NCBI Official Symbol: | RPE |
| NCBI Official Synonym Symbols: | RPE2-1 |
| NCBI Protein Information: | ribulose-phosphate 3-epimerase |
| UniProt Protein Name: | Ribulose-phosphate 3-epimerase |
| UniProt Synonym Protein Names: | Ribulose-5-phosphate-3-epimerase |
| Protein Family: | Ribulose-phosphate 3-epimerase |
| UniProt Gene Name: | RPE |
| UniProt Entry Name: | RPE_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RPE antibody (CAB14778) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunofluorescence analysis of L929 cells using RPE Polyclonal Antibody (CAB14778) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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