Cell Biology Antibodies 5

Anti-RPAP3 Antibody (CAB15239)

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SKU:
CAB15239
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-RPAP3 Antibody
Antibody SKU:CAB15239
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 17-241 of human RPAP3 (NP_001139547).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, Jurkat, 293T, HT-29, Rat testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 17-241 of human RPAP3 (NP_001139547).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:NAEE LQDF MRDL ENWE KDIK QKDM ELRR QNGV PEEN LPPI RNGN FRKK KKGK AKES SKKT REEN TKNR IKSY DYEA WAKL DVDR ILDE LDKD DSTH ESLS QESE SEED GIHV DSQK ALVL KEKG NKYF KQGK YDEA IDCY TKGM DADP YNPV LPTN RASA YFRL KKFA VAES DCNL AVAL NRSY TKAY SRRG AARF ALQK LEEA KKDY ERVL ELEP NNFE ATNE L
Gene ID:79657
Uniprot:Q9H6T3
Cellular Location:
Calculated MW:57kDa/71kDa/75kDa
Observed MW:76kDa
Synonyms:RPAP3, hSpagh
Background:This gene encodes an RNA polymerase II-associated protein. The encoded protein may function in transcriptional regulation and may also regulate apoptosis. Alternatively spliced transcript variants have been described.
UniProt Protein Function:RPAP3: Forms an interface between the RNA polymerase II enzyme and chaperone/scaffolding protein, suggesting that it is required to connect RNA polymerase II to regulators of protein complex formation. Belongs to the RPAP3 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Unknown function

Chromosomal Location of Human Ortholog: 12q13.11

Molecular Function:protein binding

NCBI Summary:This gene encodes an RNA polymerase II-associated protein. The encoded protein may function in transcriptional regulation and may also regulate apoptosis. Alternatively spliced transcript variants have been described. [provided by RefSeq, Mar 2009]
UniProt Code:Q9H6T3
NCBI GenInfo Identifier:158564023
NCBI Gene ID:79657
NCBI Accession:Q9H6T3.2
UniProt Secondary Accession:Q9H6T3,Q6PHR5, B4DRW9,
UniProt Related Accession:Q9H6T3
Molecular Weight:57,108 Da
NCBI Full Name:RNA polymerase II-associated protein 3
NCBI Synonym Full Names:RNA polymerase II associated protein 3
NCBI Official Symbol:RPAP3  
NCBI Protein Information:RNA polymerase II-associated protein 3
UniProt Protein Name:RNA polymerase II-associated protein 3
Protein Family:RNA polymerase II-associated protein
UniProt Gene Name:RPAP3  
UniProt Entry Name:RPAP3_HUMAN
Anti-RPAP3 Antibody (CAB15239)Western blot analysis of extracts of various cell lines, using RPAP3 antibody (CAB15239) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-RPAP3 Antibody (CAB15239)Immunofluorescence analysis of HeLa cells using RPAP3 antibody (CAB15239) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-RPAP3 Antibody (CAB15239)Immunofluorescence analysis of L929 cells using RPAP3 antibody (CAB15239) at dilution of 1:100. Blue: DAPI for nuclear staining.
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