Epigenetics & Nuclear Signaling Antibodies 4
Anti-RPA3 Antibody (CAB6721)
- SKU:
- CAB6721
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-RPA3 Antibody |
| Antibody SKU: | CAB6721 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 6-121 of human RPA3 (NP_002938.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse heart, Rat heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 6-121 of human RPA3 (NP_002938.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | DLPR SRIN AGML AQFI DKPV CFVG RLEK IHPT GKMF ILSD GEGK NGTI ELME PLDE EISG IVEV VGRV TAKA TILC TSYV QFKE DSHP FDLG LYNE AVKI IHDF PQFY PLGI VQHD |
| Gene ID: | 6119 |
| Uniprot: | P35244 |
| Cellular Location: | Nucleus |
| Calculated MW: | 13kDa |
| Observed MW: | 16kDa |
| Synonyms: | RPA3, REPA3, RP-A p14, RP-Ap14 |
| Background: |
| UniProt Protein Function: | RPA3: Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 7p22 Cellular Component: nucleoplasm; DNA replication factor A complex Molecular Function:protein binding; damaged DNA binding; single-stranded DNA binding Biological Process: mismatch repair; regulation of mitotic cell cycle; DNA strand elongation during DNA replication; DNA repair; double-strand break repair via homologous recombination; regulation of cell proliferation; telomere maintenance via semi-conservative replication; DNA recombinase assembly; nucleotide-excision repair; base-excision repair; double-strand break repair; transcription-coupled nucleotide-excision repair; telomere maintenance via recombination; nucleotide-excision repair, DNA damage removal; nucleotide-excision repair, DNA gap filling; mitotic cell cycle; DNA replication; telomere maintenance; G1/S transition of mitotic cell cycle |
| UniProt Code: | P35244 |
| NCBI GenInfo Identifier: | 464608 |
| NCBI Gene ID: | 6119 |
| NCBI Accession: | P35244.1 |
| UniProt Secondary Accession: | P35244,Q549U6, |
| UniProt Related Accession: | P35244 |
| Molecular Weight: | 121 |
| NCBI Full Name: | Replication protein A 14 kDa subunit |
| NCBI Synonym Full Names: | replication protein A3, 14kDa |
| NCBI Official Symbol: | RPA3 |
| NCBI Official Synonym Symbols: | REPA3 |
| NCBI Protein Information: | replication protein A 14 kDa subunit; RP-A p14; RF-A protein 3; replication factor A protein 3 |
| UniProt Protein Name: | Replication protein A 14 kDa subunit |
| UniProt Synonym Protein Names: | Replication factor A protein 3; RF-A protein 3 |
| Protein Family: | Replication protein |
| UniProt Gene Name: | RPA3 |
| UniProt Entry Name: | RFA3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RPA3 antibody (CAB6721) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunofluorescence analysis of MCF7 cells using RPA3 antibody (CAB6721). |
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