Epigenetics & Nuclear Signaling Antibodies 3
Anti-RPA2 Antibody (CAB2189)
- SKU:
- CAB2189
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-RPA2 Antibody |
| Antibody SKU: | CAB2189 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 80-270 of human RPA2 (NP_002937.1). |
| Application: | WB IHC IF IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 IP 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, 293T+UV, NIH/3T3, C6 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 80-270 of human RPA2 (NP_002937.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | IRHA EKAP TNIV YKID DMTA APMD VRQW VDTD DTSS ENTV VPPE TYVK VAGH LRSF QNKK SLVA FKIM PLED MNEF TTHI LEVI NAHM VLSK ANSQ PSAG RAPI SNPG MSEA GNFG GNSF MPAN GLTV AQNQ VLNL IKAC PRPE GLNF QDLK NQLK HMSV SSIK QAVD FLSN EGHI YSTV DDDH FKST DAE |
| Gene ID: | 6118 |
| Uniprot: | P15927 |
| Cellular Location: | Nucleus, PML body |
| Calculated MW: | 29kDa/30kDa/38kDa |
| Observed MW: | 32kDa |
| Synonyms: | REPA2, RP-A p32, RP-A p34, RPA32, RPA2, RP-Ap32, RP-Ap34 |
| Background: |
| UniProt Protein Function: | RFA2: Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Required for the efficient recruitment of the DNA double-strand break repair factor RAD51 to chromatin in response to DNA damage. Heterotrimer of 70, 32 and 14 kDa chains (canonical replication protein A complex). Component of the alternative replication protein A complex (aRPA) composed of RPA1, RPA3 and RPA4. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Binds to SERTAD3/RBT1. Interacts with TIPIN. Directly interacts with PPP4R2, but not with SMEK2; this interaction is DNA damage-dependent and leads RPA2 dephosphorylation by PPP4C recruitment. Interacts with RAD51, preferentially when hyperphosphorylated. Directly interacts with RFWD3. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:DNA replication Chromosomal Location of Human Ortholog: 1p35 Cellular Component: chromatin; chromosome, telomeric region; DNA replication factor A complex; nuclear chromosome, telomeric region; nucleoplasm; nucleus; PML body Molecular Function:damaged DNA binding; enzyme binding; protein binding; protein N-terminus binding; protein phosphatase binding; single-stranded DNA binding; ubiquitin protein ligase binding Biological Process: base-excision repair; bypass DNA synthesis; DNA damage response, detection of DNA damage; DNA repair; DNA replication; DNA strand elongation during DNA replication; double-strand break repair; double-strand break repair via homologous recombination; error-prone postreplication DNA repair; G1 DNA damage checkpoint; G1/S transition of mitotic cell cycle; mismatch repair; mitotic cell cycle; nucleotide-excision repair; nucleotide-excision repair, DNA gap filling; nucleotide-excision repair, DNA incision; telomere maintenance; telomere maintenance via recombination; telomere maintenance via semi-conservative replication; transcription-coupled nucleotide-excision repair |
| UniProt Code: | P15927 |
| NCBI GenInfo Identifier: | 132474 |
| NCBI Gene ID: | 6118 |
| NCBI Accession: | P15927.1 |
| UniProt Secondary Accession: | P15927,Q52II0, Q5TEI9, Q5TEJ5, |
| UniProt Related Accession: | P15927 |
| Molecular Weight: | 38,810 Da |
| NCBI Full Name: | Replication protein A 32 kDa subunit |
| NCBI Synonym Full Names: | replication protein A2 |
| NCBI Official Symbol: | RPA2 |
| NCBI Official Synonym Symbols: | REPA2; RPA32; RP-A p32; RP-A p34 |
| NCBI Protein Information: | replication protein A 32 kDa subunit |
| UniProt Protein Name: | Replication protein A 32 kDa subunit |
| UniProt Synonym Protein Names: | Replication factor A protein 2; RF-A protein 2; Replication protein A 34 kDa subunit; RP-A p34 |
| Protein Family: | Replication protein |
| UniProt Gene Name: | RPA2 |
| UniProt Entry Name: | RFA2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RPA2 antibody (CAB2189) at 1:1000 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded human kidney cancer using RPA2 Antibody (CAB2189) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using RPA2 antibody (CAB2189).Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 200ug extracts of Jurkat cells using 1ug RPA2 antibody (CAB2189). Western blot was performed from the immunoprecipitate using RPA2 antibody (CAB2189) at a dilition of 1:1000. |
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