Epigenetics & Nuclear Signaling Antibodies 1
Anti-RPA1 Antibody (CAB0990)
- SKU:
- CAB0990
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-RPA1 Antibody |
| Antibody SKU: | CAB0990 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF IP |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 367-616 of human RPA1 (NP_002936.1). |
| Application: | WB IF IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 IP 1:50 - 1:100 |
| Reactivity: | Human, Mouse |
| Positive Samples: | U-87MG, Mouse spleen |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 367-616 of human RPA1 (NP_002936.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | KFDG SRQP VLAI KGAR VSDF GGRS LSVL SSST IIAN PDIP EAYK LRGW FDAE GQAL DGVS ISDL KSGG VGGS NTNW KTLY EVKS ENLG QGDK PDYF SSVA TVVY LRKE NCMY QACP TQDC NKKV IDQQ NGLY RCEK CDTE FPNF KYRM ILSV NIAD FQEN QWVT CFQE SAEA ILGQ NAAY LGEL KDKN EQAF EEVF QNAN FRSF IFRV RVKV ETYN DESR IKAT VMDV KPVD YREY GRRL VMSI RRSA LM |
| Gene ID: | 6117 |
| Uniprot: | P27694 |
| Cellular Location: | Nucleus, PML body |
| Calculated MW: | 68kDa |
| Observed MW: | 68kDa |
| Synonyms: | RPA1, HSSB, MST075, REPA1, RF-A, RP-A, RPA70 |
| Background: |
| UniProt Protein Function: | RPA1: Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing. Heterotrimer composed of RPA1, RPA2 and RPA3 (canonical replication protein A complex). Component of the alternative replication protein A complex (aRPA) composed of RPA1, RPA3 and RPA4. The DNA-binding activity may reside exclusively on the RPA1 subunit. Interacts with RIPK1 and XPA. Interacts with RPA4. Interacts with the polymerase alpha subunit POLA1/p180; this interaction stabilizes the replicative complex and reduces the misincorporation rate of DNA polymerase alpha by acting as a fidelity clamp. Interact with RAD51 and SENP6 to regulate DNA repair. Interacts with HELB; this interaction promotes HELB recruitement to chromatin following DNA damage. Belongs to the replication factor A protein 1 family. |
| UniProt Protein Details: | Protein type:DNA-binding Chromosomal Location of Human Ortholog: 17p13.3 Cellular Component: male germ cell nucleus; nucleoplasm; lateral element; PML body; DNA replication factor A complex; nucleus Molecular Function:protein binding; metal ion binding; damaged DNA binding; chromatin binding; single-stranded DNA binding Biological Process: meiosis; mismatch repair; in utero embryonic development; DNA strand elongation during DNA replication; DNA repair; double-strand break repair via homologous recombination; DNA recombination; telomere maintenance via semi-conservative replication; DNA recombinase assembly; homeostasis of number of cells within a tissue; base-excision repair; nucleotide-excision repair; double-strand break repair; transcription-coupled nucleotide-excision repair; positive regulation of cell proliferation; telomere maintenance via recombination; hemopoiesis; nucleotide-excision repair, DNA damage removal; nucleotide-excision repair, DNA gap filling; mitotic cell cycle; DNA replication; DNA-dependent DNA replication; telomere maintenance; G1/S transition of mitotic cell cycle |
| UniProt Code: | P27694 |
| NCBI GenInfo Identifier: | 1350579 |
| NCBI Gene ID: | 6117 |
| NCBI Accession: | P27694.2 |
| UniProt Secondary Accession: | P27694,Q59ES9, A8K0Y9, |
| UniProt Related Accession: | P27694 |
| Molecular Weight: | 616 |
| NCBI Full Name: | Replication protein A 70 kDa DNA-binding subunit |
| NCBI Synonym Full Names: | replication protein A1, 70kDa |
| NCBI Official Symbol: | RPA1 |
| NCBI Official Synonym Symbols: | HSSB; RF-A; RP-A; REPA1; RPA70; MST075 |
| NCBI Protein Information: | replication protein A 70 kDa DNA-binding subunit; MSTP075; RP-A p70; RF-A protein 1; replication factor A protein 1; single-stranded DNA-binding protein |
| UniProt Protein Name: | Replication protein A 70 kDa DNA-binding subunit |
| UniProt Synonym Protein Names: | Replication factor A protein 1; RF-A protein 1; Single-stranded DNA-binding proteinReplication protein A 70 kDa DNA-binding subunit, N-terminally processed |
| Protein Family: | Replication factor |
| UniProt Gene Name: | RPA1 |
| UniProt Entry Name: | RFA1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RPA1 antibody (CAB0990) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of MCF-7 cells using RPA1 antibody (CAB0990). Blue: DAPI for nuclear staining. |
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