Cell Biology Antibodies 5
Anti-ROBO3 Antibody (CAB14922)
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- SKU:
- CAB14922
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-ROBO3 Antibody |
| Antibody SKU: | CAB14922 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human ROBO3 (NP_071765.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | Jurkat, SP2/0, SGC-7901 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human ROBO3 (NP_071765.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MLRY LLKT LLQM NLFA DSLA GDIS NSSE LLLG FNSS LAAL NHTL LPPG DPSL NGSR VGPE DAMP RIVE QPPD LLVS RGEP ATLP CRAE GRPR PNIE WYKN GARV ATVR EDPR AHRL LLPS GALF FPRI VHGR RARP DEGV YTCV ARN |
| Gene ID: | 64221 |
| Uniprot: | Q96MS0 |
| Cellular Location: | Membrane, Single-pass type I membrane protein |
| Calculated MW: | 110kDa/148kDa |
| Observed MW: | 148kDa |
| Synonyms: | ROBO3, HGPPS, HGPS, RBIG1, RIG1 |
| Background: | This gene is a member of the Roundabout (ROBO) gene family that controls neurite outgrowth, growth cone guidance, and axon fasciculation. ROBO proteins are a subfamily of the immunoglobulin transmembrane receptor superfamily. SLIT proteins 1-3, a family of secreted chemorepellants, are ligands for ROBO proteins and SLIT/ROBO interactions regulate myogenesis, leukocyte migration, kidney morphogenesis, angiogenesis, and vasculogenesis in addition to neurogenesis. This gene, ROBO3, has a putative extracellular domain with five immunoglobulin (Ig)-like loops and three fibronectin (Fn) type III motifs, a transmembrane segment, and a cytoplasmic tail with three conserved signaling motifs: CC0, CC2, and CC3 (CC for conserved cytoplasmic). Unlike other ROBO family members, ROBO3 lacks motif CC1. The ROBO3 gene regulates axonal navigation at the ventral midline of the neural tube. In mouse, loss of Robo3 results in a complete failure of commissural axons to cross the midline throughout the spinal cord and the hindbrain. Mutations ROBO3 result in horizontal gaze palsy with progressive scoliosis (HGPPS); an autosomal recessive disorder characterized by congenital absence of horizontal gaze, progressive scoliosis, and failure of the corticospinal and somatosensory axon tracts to cross the midline in the medulla. Alternative transcript variants have been described but have not been experimentally validated. |
| UniProt Protein Function: | ROBO3: Thought to be involved during neural development in axonal navigation at the ventral midline of the neural tube. In spinal chord development plays a role in guiding commissural axons probably by preventing premature sensitivity to Slit proteins thus inhibiting Slit signaling through ROBO1. Required for hindbrain axon midline crossing. Defects in ROBO3 are a cause of familial horizontal gaze palsy with progressive scoliosis (HGPPS). Patients show a medulla where motor and sensory projections appear uncrossed. Belongs to the immunoglobulin superfamily. ROBO family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 11q24.2 Cellular Component: axon; integral to membrane Biological Process: axon guidance; neuron migration; axon midline choice point recognition Disease: Gaze Palsy, Familial Horizontal, With Progressive Scoliosis |
| NCBI Summary: | This gene is a member of the Roundabout (ROBO) gene family that controls neurite outgrowth, growth cone guidance, and axon fasciculation. ROBO proteins are a subfamily of the immunoglobulin transmembrane receptor superfamily. SLIT proteins 1-3, a family of secreted chemorepellants, are ligands for ROBO proteins and SLIT/ROBO interactions regulate myogenesis, leukocyte migration, kidney morphogenesis, angiogenesis, and vasculogenesis in addition to neurogenesis. This gene, ROBO3, has a putative extracellular domain with five immunoglobulin (Ig)-like loops and three fibronectin (Fn) type III motifs, a transmembrane segment, and a cytoplasmic tail with three conserved signaling motifs: CC0, CC2, and CC3 (CC for conserved cytoplasmic). Unlike other ROBO family members, ROBO3 lacks motif CC1. The ROBO3 gene regulates axonal navigation at the ventral midline of the neural tube. In mouse, loss of Robo3 results in a complete failure of commissural axons to cross the midline throughout the spinal cord and the hindbrain. Mutations ROBO3 result in horizontal gaze palsy with progressive scoliosis (HGPPS); an autosomal recessive disorder characterized by congenital absence of horizontal gaze, progressive scoliosis, and failure of the corticospinal and somatosensory axon tracts to cross the midline in the medulla. Alternative transcript variants have been described but have not been experimentally validated. [provided by RefSeq, Dec 2009] |
| UniProt Code: | Q96MS0 |
| NCBI GenInfo Identifier: | 119588006 |
| NCBI Gene ID: | 64221 |
| NCBI Accession: | EAW67602.1 |
| UniProt Related Accession: | Q96MS0 |
| Molecular Weight: | 110,676 Da |
| NCBI Full Name: | roundabout, axon guidance receptor, homolog 3 (Drosophila), isoform CRA_a |
| NCBI Synonym Full Names: | roundabout, axon guidance receptor, homolog 3 (Drosophila) |
| NCBI Official Symbol: | ROBO3 |
| NCBI Official Synonym Symbols: | HGPS; RIG1; HGPPS; RBIG1 |
| NCBI Protein Information: | roundabout homolog 3; roundabout-like protein 3; retinoblastoma inhibiting gene 1 |
| UniProt Protein Name: | Roundabout homolog 3 |
| UniProt Synonym Protein Names: | Roundabout-like protein 3 |
| Protein Family: | Roundabout |
| UniProt Gene Name: | ROBO3 |
| UniProt Entry Name: | ROBO3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ROBO3 antibody (CAB14922) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
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