Cell Biology Antibodies 9
Anti-RNPEP Antibody (CAB4080)
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- SKU:
- CAB4080
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-RNPEP Antibody |
| Antibody SKU: | CAB4080 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 371-650 of human RNPEP (NP_064601.3). |
| Application: | WB |
| Recommended Dilution: | WB 1:1000 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | NCI-H460, U-251MG, HepG2, HeLa, HL-60, Mouse kidney, Mouse brain, Rat testis, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 371-650 of human RNPEP (NP_064601.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | EAAT GRAL LRQH MDIT GEEN PLNK LRVK IEPG VDPD DTYN ETPY EKGF CFVS YLAH LVGD QDQF DSFL KAYV HEFK FRSI LADD FLDF YLEY FPEL KKKR VDII PGFE FDRW LNTP GWPP YLPD LSPG DSLM KPAE ELAQ LWAA EELD MKAI EAVA ISPW KTYQ LVYF LDKI LQKS PLPP GNVK KLGD TYPS ISNA RNAE LRLR WGQI VLKN DHQE DFWK VKEF LHNQ GKQK YTLP LYHA MMGG SEVA QTLA KETF ASTA SQLH SNVV NYVQ QIVA PKGS |
| Gene ID: | 6051 |
| Uniprot: | Q9H4A4 |
| Cellular Location: | Secreted |
| Calculated MW: | 72kDa |
| Observed MW: | 80kDa |
| Synonyms: | RNPEP, AP-B, APB |
| Background: |
| UniProt Protein Function: | RNPEP: Exopeptidase which selectively removes arginine and/or lysine residues from the N-terminus of several peptide substrates including Arg(0)-Leu-enkephalin, Arg(0)-Met-enkephalin and Arg(- 1)-Lys(0)-somatostatin-14. Can hydrolyze leukotriene A4 (LTA-4) into leukotriene B4 (LTB-4). Belongs to the peptidase M1 family. |
| UniProt Protein Details: | Protein type:EC 3.4.11.6; Protease Chromosomal Location of Human Ortholog: 1q32 Cellular Component: extracellular space; plasma membrane; extracellular region Molecular Function:metalloexopeptidase activity; zinc ion binding; epoxide hydrolase activity; aminopeptidase activity Biological Process: leukotriene biosynthetic process; proteolysis |
| UniProt Code: | Q9H4A4 |
| NCBI GenInfo Identifier: | 20137480 |
| NCBI Gene ID: | 6051 |
| NCBI Accession: | Q9H4A4.2 |
| UniProt Secondary Accession: | Q9H4A4,Q8VCT3, O09175, |
| UniProt Related Accession: | Q9H4A4 |
| Molecular Weight: | 650 |
| NCBI Full Name: | Aminopeptidase B |
| NCBI Synonym Full Names: | arginyl aminopeptidase (aminopeptidase B) |
| NCBI Official Symbol: | RNPEP |
| NCBI Protein Information: | aminopeptidase B; AP-B; arginine aminopeptidase |
| UniProt Protein Name: | Aminopeptidase B |
| UniProt Synonym Protein Names: | Arginine aminopeptidase; Arginyl aminopeptidase |
| Protein Family: | Aminopeptidase |
| UniProt Gene Name: | RNPEP |
| UniProt Entry Name: | AMPB_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RNPEP antibody (CAB4080) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
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