Epigenetics & Nuclear Signaling Antibodies 2

Anti-RNPC3 Antibody (CAB6333)

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SKU:
CAB6333
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-RNPC3 Antibody
Antibody SKU:CAB6333
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 258-517 of human RNPC3 (NP_060089.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, 293T, MCF7, Mouse kidney, Rat kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 258-517 of human RNPC3 (NP_060089.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:NKLM ELAN LQPK RPKT IKQR HVRK KRKI KDML NTPL CPSH SSLH PVLL PSDV FDQP QPVG NKRI EFHI STDM PAAF KKDL EKEQ NCEE KNHD LPAT EVDA SNIG FGKI FPKP NLDI TEEI KEDS DEMP SECI SRRE LEKG RISR EEME TLSV FRSY EPGE PNCR IYVK NLAK HVQE KDLK YIFG RYVD FSSE TQRI MFDI RLMK EGRM KGQA FIGL PNEK AAAK ALKE ANGY VLFG KPMV VQFA RSAR PKQD PKEG KRKC
Gene ID:55599
Uniprot:Q96LT9
Cellular Location:Nucleus
Calculated MW:58kDa
Observed MW:60-75kDa
Synonyms:RNPC3, RBM40, RNP, SNRNP65
Background:Two types of spliceosomes catalyze splicing of pre-mRNAs. The major U2-type spliceosome is found in all eukaryotes and removes U2-type introns, which represent more than 99% of pre-mRNA introns. The minor U12-type spliceosome is found in some eukaryotes and removes U12-type introns, which are rare and have distinct splice consensus signals. The U12-type spliceosome consists of several small nuclear RNAs and associated proteins. This gene encodes a 65K protein that is a component of the U12-type spliceosome. This protein contains two RNA recognition motifs (RRMs), suggesting that it may contact one of the small nuclear RNAs of the minor spliceosome.
UniProt Protein Function:RNPC3: Participates in pre-mRNA U12-dependent splicing, performed by the minor spliceosome which removes U12-type introns. U12-type introns comprises less than 1% of all non-coding sequences. Binds to the 3'-stem-loop of m(7)G-capped U12 snRNA.
UniProt Protein Details:

Protein type:RNA splicing

Chromosomal Location of Human Ortholog: 1p21

Cellular Component: nucleoplasm; nucleus; U12-dependent spliceosome

Molecular Function:RNA binding; nucleotide binding

Biological Process: RNA splicing; mRNA processing

NCBI Summary:Two types of spliceosomes catalyze splicing of pre-mRNAs. The major U2-type spliceosome is found in all eukaryotes and removes U2-type introns, which represent more than 99% of pre-mRNA introns. The minor U12-type spliceosome is found in some eukaryotes and removes U12-type introns, which are rare and have distinct splice consensus signals. The U12-type spliceosome consists of several small nuclear RNAs and associated proteins. This gene encodes a 65K protein that is a component of the U12-type spliceosome. This protein contains two RNA recognition motifs (RRMs), suggesting that it may contact one of the small nuclear RNAs of the minor spliceosome. [provided by RefSeq, Jul 2008]
UniProt Code:Q96LT9
NCBI GenInfo Identifier:74760946
NCBI Gene ID:55599
NCBI Accession:Q96LT9.1
UniProt Secondary Accession:Q96LT9,Q5TZ87, Q96FK7, Q96JI8, Q9NSU7, Q9NXX2, A8K1C9 D3DT74,
UniProt Related Accession:Q96LT9
Molecular Weight:Calculated: 58 kDaObserved: 60-75 kDa
NCBI Full Name:RNA-binding protein 40
NCBI Synonym Full Names:RNA-binding region (RNP1, RRM) containing 3
NCBI Official Symbol:RNPC3  
NCBI Official Synonym Symbols:RNP; RBM40; SNRNP65  
NCBI Protein Information:RNA-binding protein 40; U11/U12-65K; U11/U12 snRNP 65K; RNA recognition protein; RNA-binding motif protein 40; U11/U12 snRNP 65 kDa protein; RNA-binding region-containing protein 3; U11/U12 small nuclear ribonucleoprotein 65 kDa protein
UniProt Protein Name:RNA-binding protein 40
UniProt Synonym Protein Names:RNA-binding motif protein 40; RNA-binding region-containing protein 3; U11/U12 small nuclear ribonucleoprotein 65 kDa protein; U11/U12 snRNP 65 kDa protein; U11/U12-65K
Protein Family:RNA-binding protein
UniProt Gene Name:RNPC3  
UniProt Entry Name:RBM40_HUMAN
Anti-RNPC3 Antibody (CAB6333)Western blot analysis of extracts of various cell lines, using RNPC3 antibody (CAB6333) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 60s.
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