Cell Biology Antibodies 9
Anti-RNH1 Antibody (CAB4079)
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- SKU:
- CAB4079
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-RNH1 Antibody |
| Antibody SKU: | CAB4079 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 202-461 of human RNH1 (NP_002930.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:1000 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Raji, U-87MG, HT-29, HeLa, Mouse liver, Mouse kidney, Mouse lung, Rat brain, Rat lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 202-461 of human RNH1 (NP_002930.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | EALK LESC GVTS DNCR DLCG IVAS KASL RELA LGSN KLGD VGMA ELCP GLLH PSSR LRTL WIWE CGIT AKGC GDLC RVLR AKES LKEL SLAG NELG DEGA RLLC ETLL EPGC QLES LWVK SCSF TAAC CSHF SSVL AQNR FLLE LQIS NNRL EDAG VREL CQGL GQPG SVLR VLWL ADCD VSDS SCSS LAAT LLAN HSLR ELDL SNNC LGDA GILQ LVES VRQP GCLL EQLV LYDI YWSE EMED RLQA LEKD KPSL RVIS |
| Gene ID: | 6050 |
| Uniprot: | P13489 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 49kDa |
| Observed MW: | 43kDa |
| Synonyms: | RNH1, RAI, RNH |
| Background: | Placental ribonuclease inhibitor (PRI) is a member of a family of proteinaceous cytoplasmic RNase inhibitors that occur in many tissues and bind to both intracellular and extracellular RNases (summarized by Lee et al., 1988 [PubMed 3219362]). In addition to control of intracellular RNases, the inhibitor may have a role in the regulation of angiogenin (MIM 105850). Ribonuclease inhibitor, of 50, 000 Da, binds to ribonucleases and holds them in a latent form. Since neutral and alkaline ribonucleases probably play a critical role in the turnover of RNA in eukaryotic cells, RNH may be essential for control of mRNA turnover; the interaction of eukaryotic cells with ribonuclease may be reversible in vivo.[supplied by OMIM, Jul 2010] |
| UniProt Protein Function: | RNH1: Ribonuclease inhibitor which inhibits RNASE1, RNASE2 and ANG. May play a role in redox homeostasis. |
| UniProt Protein Details: | Protein type:Inhibitor Chromosomal Location of Human Ortholog: 11p15.5 Cellular Component: angiogenin-PRI complex; cytoplasm Molecular Function:protein binding; ribonuclease inhibitor activity Biological Process: negative regulation of catalytic activity; mRNA catabolic process; regulation of angiogenesis |
| NCBI Summary: | Placental ribonuclease inhibitor (PRI) is a member of a family of proteinaceous cytoplasmic RNase inhibitors that occur in many tissues and bind to both intracellular and extracellular RNases (summarized by Lee et al., 1988 [PubMed 3219362]). In addition to control of intracellular RNases, the inhibitor may have a role in the regulation of angiogenin (MIM 105850). Ribonuclease inhibitor, of 50,000 Da, binds to ribonucleases and holds them in a latent form. Since neutral and alkaline ribonucleases probably play a critical role in the turnover of RNA in eukaryotic cells, RNH may be essential for control of mRNA turnover; the interaction of eukaryotic cells with ribonuclease may be reversible in vivo.[supplied by OMIM, Jul 2010] |
| UniProt Code: | P13489 |
| NCBI GenInfo Identifier: | 132573 |
| NCBI Gene ID: | 6050 |
| NCBI Accession: | P13489.2 |
| UniProt Secondary Accession: | P13489,Q8IZK8, Q96FD7, Q9BQ80, Q9UDK6, |
| UniProt Related Accession: | P13489 |
| Molecular Weight: | |
| NCBI Full Name: | Ribonuclease inhibitor |
| NCBI Synonym Full Names: | ribonuclease/angiogenin inhibitor 1 |
| NCBI Official Symbol: | RNH1 |
| NCBI Official Synonym Symbols: | RAI; RNH |
| NCBI Protein Information: | ribonuclease inhibitor; placental RNase inhibitor; placental ribonuclease inhibitor |
| UniProt Protein Name: | Ribonuclease inhibitor |
| UniProt Synonym Protein Names: | Placental ribonuclease inhibitor; Placental RNase inhibitor; Ribonuclease/angiogenin inhibitor 1; RAI |
| Protein Family: | Ribonuclease inhibitor |
| UniProt Gene Name: | RNH1 |
| UniProt Entry Name: | RINI_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RNH1 antibody (CAB4079) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of C6 cells using RNH1 Rabbit pAb (CAB4079) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of L929 cells using RNH1 Rabbit pAb (CAB4079) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using RNH1 Rabbit pAb (CAB4079) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining. |
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