Name: Anti-RNF8 Antibody (CAB7302)
Brand: Cell Cycle Antibodies 2
SKU: CAB7302
Availability: OutOfStock

Antibody Name:	Anti-RNF8 Antibody
Antibody SKU:	CAB7302
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human RNF8 (NP_003949.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human RNF8 (NP_003949.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MGEP GFFV TGDR AGGR SWCL RRVG MSAG WLLL EDGC EVTV GRGF GVTY QLVS KICP LMIS RNHC VLKQ NPEG QWTI MDNK SLNG VWLN RARL EPLR VYSI HQGD YIQL GVPL ENKE NAEY EYEV TEED WETI YPCL SPKN DQMI EKNK ELRT KRKF SLDE LAGP GAEG PSNL KSKI NKVS CESG QPVK SQGK GEVA STPS DNLD PKLT ALEP SKTT GAPI YPGF PKVT EVHH EQKA SNSS ASQR SLQM FKVT MSRI LRLK
Gene ID:	9025
Uniprot:	O76064
Cellular Location:	Chromosome, Midbody, Nucleus, telomere
Calculated MW:	10kDa/50kDa/55kDa
Observed MW:	68kDa

Synonyms:

RNF8, hRNF8

Background:

The protein encoded by this gene contains a RING finger motif and an FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating enzymes (E2), including UBE2E1/UBCH6, UBE2E2, and UBE2E3, and may act as an ubiquitin ligase (E3) in the ubiquitination of certain nuclear proteins. This protein is also known to play a role in the DNA damage response and depletion of this protein causes cell growth inhibition and cell cycle arrest. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	RNF8: E3 ubiquitin-protein ligase that plays a key role in DNA damage signaling via 2 distinct roles: by mediating the 'Lys-63'- linked ubiquitination of histones H2A and H2AX and promoting the recruitment of DNA repair proteins at double-strand breaks (DSBs) sites, and by catalyzing 'Lys-48'-linked ubiquitination to remove target proteins from DNA damage sites. Following DNA DSBs, it is recruited to the sites of damage by ATM-phosphorylated MDC1 and catalyzes the 'Lys-63'-linked ubiquitination of histones H2A and H2AX, thereby promoting the formation of TP53BP1 and BRCA1 ionizing radiation-induced foci (IRIF). Also controls the recruitment of UIMC1-BRCC3 (RAP80-BRCC36) and PAXIP1/PTIP to DNA damage sites. Also recruited at DNA interstrand cross-links (ICLs) sites and catalyzes 'Lys-63'-linked ubiquitination of histones H2A and H2AX, leading to recruitment of FAAP20/C1orf86 and Fanconi anemia (FA) complex, followed by interstrand cross-link repair. H2A ubiquitination also mediates the ATM-dependent transcriptional silencing at regions flanking DSBs in cis, a mechanism to avoid collision between transcription and repair intermediates. Promotes the formation of 'Lys-63'-linked polyubiquitin chains via interactions with the specific ubiquitin-conjugating UBE2N/UBC13 and ubiquitinates non-histone substrates such as PCNA. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation. Also catalyzes the formation of 'Lys-48'-linked polyubiquitin chains via interaction with the ubiquitin- conjugating UBE2L6/UBCH8, leading to degradation of substrate proteins such as CHEK2, JMJD2A/KDM4A and KU80/XRCC5: it is still unclear how the preference toward 'Lys-48'- versus 'Lys-63'-linked ubiquitination is regulated but it could be due to RNF8 ability to interact with specific E2 specific ligases. For instance, interaction with phosphorylated HERC2 promotes the association between RNF8 and UBE2N/UBC13 and favors the specific formation of 'Lys-63'-linked ubiquitin chains. Promotes non-homologous end joining (NHEJ) by promoting the 'Lys-48'-linked ubiquitination and degradation the of KU80/XRCC5. Following DNA damage, mediates the ubiquitination and degradation of JMJD2A/KDM4A in collaboration with RNF168, leading to unmask H4K20me2 mark and promote the recruitment of TP53BP1 at DNA damage sites. In addition to its function in damage signaling, also plays a role in higher-order chromatin structure by mediating extensive chromatin decondensation. Involved in the activation of ATM by promoting histone H2B ubiquitination, which indirectly triggers histone H4 'Lys-16' acetylation (H4K16ac), establishing a chromatin environment that promotes efficient activation of ATM kinase. Required in the testis, where it plays a role in the replacement of histones during spermatogenesis. At uncapped telomeres, promotes the joining of deprotected chromosome ends by inducing H2A ubiquitination and TP53BP1 recruitment, suggesting that it may enhance cancer development by aggraving telomere-induced genome instability in case of telomeric crisis. Promotes the assembly of RAD51 at DNA DSBs in the absence of BRCA1 and TP53BP1 Also involved in class switch recombination in immune system, via its role in regulation of DSBs repair. May be required for proper exit from mitosis after spindle checkpoint activation and may regulate cytokinesis. May play a role in the regulation of RXRA-mediated transcriptional activity. Not involved in RXRA ubiquitination by UBE2E2. Belongs to the RNF8 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Ligase; Transcription, coactivator/corepressor; EC 6.3.2.19; DNA repair, damage; Nuclear receptor co-regulator; EC 6.3.2.-; Histone-binding; Ubiquitin ligase; Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 6p21.3 Cellular Component: chromosome, telomeric region; cytoplasm; nucleoplasm; nucleus; ubiquitin ligase complex Molecular Function:chromatin binding; histone binding; protein binding; protein homodimerization activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; zinc ion binding Biological Process: double-strand break repair; double-strand break repair via nonhomologous end joining; histone exchange; histone H2A ubiquitination; histone H2B ubiquitination; isotype switching; positive regulation of DNA repair; protein autoubiquitination; response to DNA damage stimulus; response to ionizing radiation; spermatid development; ubiquitin-dependent protein catabolic process
NCBI Summary:	The protein encoded by this gene contains a RING finger motif and an FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating enzymes (E2), including UBE2E1/UBCH6, UBE2E2, and UBE2E3, and may act as an ubiquitin ligase (E3) in the ubiquitination of certain nuclear proteins. This protein is also known to play a role in the DNA damage response and depletion of this protein causes cell growth inhibition and cell cycle arrest. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]
UniProt Code:	O76064
NCBI GenInfo Identifier:	21362894
NCBI Gene ID:	9025
NCBI Accession:	O76064.1
UniProt Secondary Accession:	O76064,Q53H16, Q5NKW5, A6NN24, A8MYC0, B4DPG0,
UniProt Related Accession:	O76064
Molecular Weight:	50,829 Da
NCBI Full Name:	E3 ubiquitin-protein ligase RNF8
NCBI Synonym Full Names:	ring finger protein 8
NCBI Official Symbol:	RNF8
NCBI Official Synonym Symbols:	hRNF8
NCBI Protein Information:	E3 ubiquitin-protein ligase RNF8
UniProt Protein Name:	E3 ubiquitin-protein ligase RNF8
UniProt Synonym Protein Names:	RING finger protein 8
Protein Family:	E3 ubiquitin-protein ligase
UniProt Gene Name:	RNF8
UniProt Entry Name:	RNF8_HUMAN

Western blot analysis of extracts of HepG2 cells, using RNF8 antibody (CAB7302) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 10s.

Antibodies

Anti-RNF8 Antibody (CAB14083)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)