Name: Anti-RNF5 Antibody (CAB8351)
Brand: Signal Transduction Antibodies 3
SKU: CAB8351
Availability: OutOfStock

Antibody Name:	Anti-RNF5 Antibody
Antibody SKU:	CAB8351
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human RNF5 (NP_008844.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, Jurkat, NCI-H460, HepG2, U-251MG, Mouse liver, Mouse spleen, Mouse kidney, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human RNF5 (NP_008844.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAAA EEED GGPE GPNR ERGG AGAT FECN ICLE TARE AVVS VCGH LYCW PCLH QWLE TRPE RQEC PVCK AGIS REKV VPLY GRGS QKPQ DPRL KTPP RPQG QRPA PESR GGFQ PFGD TGGF
Gene ID:	6048
Uniprot:	Q99942
Cellular Location:	Endoplasmic reticulum membrane, Membrane, Mitochondrion membrane, Multi-pass membrane protein
Calculated MW:	19kDa
Observed MW:	21kDa

Synonyms:	RNF5, RING5, RMA1
Background:	The protein encoded by this gene contains a RING finger, which is a motif known to be involved in protein-protein interactions. This protein is a membrane-bound ubiquitin ligase. It can regulate cell motility by targeting paxillin ubiquitination and altering the distribution and localization of paxillin in cytoplasm and cell focal adhesions.

UniProt Protein Function:	RNF5: Has E2-dependent E3 ubiquitin-protein ligase activity. May function together with E2 ubiquitin-conjugating enzymes UBE2D1/UBCH5A and UBE2D2/UBC4. Mediates ubiquitination of PXN/paxillin and Salmonella type III secreted protein sopA. May be involved in regulation of cell motility and localization of PXN/paxillin. Mediates the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD; the ubiquitination appears to involve E2 ubiquitin-conjugating enzyme UBE2N. Mediates the 'Lys-48'-linked polyubiquitination of TMEM173 at 'Lys-150' leading to its proteasomal degradation; the ubiquitination occurrs in mitochondria after viral transfection and regulates antiviral responses.
UniProt Protein Details:	Protein type:Membrane protein, integral; Ubiquitin conjugating system; Ligase; EC 6.3.2.-; EC 6.3.2.19; Ubiquitin ligase; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 6p21.3 Molecular Function:protein binding; ubiquitin-protein ligase activity Biological Process: cellular protein catabolic process
NCBI Summary:	The protein encoded by this gene contains a RING finger, which is a motif known to be involved in protein-protein interactions. This protein is a membrane-bound ubiquitin ligase. It can regulate cell motility by targeting paxillin ubiquitination and altering the distribution and localization of paxillin in cytoplasm and cell focal adhesions. [provided by RefSeq, Jul 2008]
UniProt Code:	Q99942
NCBI GenInfo Identifier:	74762702
NCBI Gene ID:	6048
NCBI Accession:	Q99942.1
UniProt Secondary Accession:	Q99942,Q0VDB7, Q9UMQ2, A2BFI6, B2R4K3,
UniProt Related Accession:	Q99942
Molecular Weight:	19,881 Da
NCBI Full Name:	E3 ubiquitin-protein ligase RNF5
NCBI Synonym Full Names:	ring finger protein 5
NCBI Official Symbol:	RNF5
NCBI Official Synonym Symbols:	RMA1; RING5
NCBI Protein Information:	E3 ubiquitin-protein ligase RNF5
UniProt Protein Name:	E3 ubiquitin-protein ligase RNF5
UniProt Synonym Protein Names:	Protein G16; RING finger protein 5; Ram1 homolog; HsRma1
Protein Family:	E3 ubiquitin-protein ligase
UniProt Gene Name:	RNF5
UniProt Entry Name:	RNF5_HUMAN

	Western blot analysis of extracts of various cell lines, using RNF5 antibody (CAB8351) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat liver using RNF5 antibody (CAB8351) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human liver cancer using RNF5 antibody (CAB8351) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse heart using RNF5 antibody (CAB8351) at dilution of 1:100 (40x lens).