Epigenetics & Nuclear Signaling Antibodies 1
Anti-RNF4 Antibody (CAB12037)
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- SKU:
- CAB12037
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-RNF4 Antibody |
| Antibody SKU: | CAB12037 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human RNF4 (NP_001171938.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:1000 |
| Reactivity: | Mouse, Rat |
| Positive Samples: | Mouse kidney, Mouse brain, Rat testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human RNF4 (NP_001171938.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSTR KRRG GAIN SRQA QKRT REAT STPE ISLE AEPI ELVE TAGD EIVD LTCE SLEP VVVD LTHN DSVV IVDE RRRP RRNA RRLP QDHA DSCV VSSD DEEL SRDR DVYV TTHT PRNA RDEG ATGL RPSG TVSC PICM DGYS EIVQ NGRL IVST ECGH VFCS QCLR DSLK NANT CPTC RKKI NHKR YHPI YI |
| Gene ID: | 6047 |
| Uniprot: | P78317 |
| Cellular Location: | Cytoplasm, Nucleus, PML body, nucleoplasm |
| Calculated MW: | 12kDa/21kDa |
| Observed MW: | 21kDa |
| Synonyms: | RNF4, RES4-26, SLX5, SNURF |
| Background: | The protein encoded by this gene contains a RING finger motif and acts as a transcription regulator. This protein has been shown to interact with, and inhibit the activity of, TRPS1, a transcription suppressor of GATA-mediated transcription. Transcription repressor ZNF278/PATZ is found to interact with this protein, and thus reduce the enhancement of androgen receptor-dependent transcription mediated by this protein. Studies of the mouse and rat counterparts suggested a role of this protein in spermatogenesis. A pseudogene of this gene is found on chromosome 1. |
| UniProt Protein Function: | RNF4: E3 ubiquitin-protein ligase which binds polysumoylated chains covalently attached to proteins and mediates 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination of those substrates and their subsequent targeting to the proteasome for degradation. Regulates the degradation of several proteins including PML and the transcriptional activator PEA3. Involved in chromosome alignment and spindle assembly, it regulates the kinetochore CENPH-CENPI-CENPK complex by targeting polysumoylated CENPI to proteasomal degradation. Regulates the cellular responses to hypoxia and heat shock through degradation of respectively EPAS1 and PARP1. Alternatively, it may also bind DNA/nucleosomes and have a more direct role in the regulation of transcription for instance enhancing basal transcription and steroid receptor- mediated transcriptional activation. |
| UniProt Protein Details: | Protein type:EC 6.3.2.-; Ligase; Nuclear receptor co-regulator; Transcription factor; Ubiquitin conjugating system; Ubiquitin ligase Chromosomal Location of Human Ortholog: 4p16.3 Cellular Component: cytoplasm; nucleoplasm; nucleus; PML body Molecular Function:DNA binding; identical protein binding; nucleosome binding; protein binding; SUMO polymer binding; transcription factor activity; ubiquitin-protein ligase activity Biological Process: positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; proteasomal ubiquitin-dependent protein catabolic process; protein autoubiquitination; protein ubiquitination; response to arsenic |
| NCBI Summary: | The protein encoded by this gene contains a RING finger motif and acts as a transcription regulator. This protein has been shown to interact with, and inhibit the activity of, TRPS1, a transcription suppressor of GATA-mediated transcription. Transcription repressor ZNF278/PATZ is found to interact with this protein, and thus reduce the enhancement of androgen receptor-dependent transcription mediated by this protein. Studies of the mouse and rat counterparts suggested a role of this protein in spermatogenesis. A pseudogene of this gene is found on chromosome 1.[provided by RefSeq, Jul 2010] |
| UniProt Code: | P78317 |
| NCBI GenInfo Identifier: | 18202358 |
| NCBI Gene ID: | 6047 |
| NCBI Accession: | P78317.1 |
| UniProt Secondary Accession: | P78317,Q49AR8, B2R6D6, D6RF58, |
| UniProt Related Accession: | P78317 |
| Molecular Weight: | 21kDa |
| NCBI Full Name: | E3 ubiquitin-protein ligase RNF4 |
| NCBI Synonym Full Names: | ring finger protein 4 |
| NCBI Official Symbol: | RNF4 |
| NCBI Official Synonym Symbols: | SLX5; SNURF; RES4-26 |
| NCBI Protein Information: | E3 ubiquitin-protein ligase RNF4 |
| UniProt Protein Name: | E3 ubiquitin-protein ligase RNF4 |
| UniProt Synonym Protein Names: | RING finger protein 4; RING-type E3 ubiquitin transferase RNF4Curated; Small nuclear ring finger protein; Protein SNURF |
| Protein Family: | E3 ubiquitin-protein ligase |
| UniProt Gene Name: | RNF4 |
 | Western blot analysis of extracts of various cell lines, using RNF4 antibody (CAB12037) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
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