Signal Transduction Antibodies 2
Anti-RNF220 Antibody (CAB15853)
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- SKU:
- CAB15853
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Description
| Antibody Name: | Anti-RNF220 Antibody |
| Antibody SKU: | CAB15853 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 267-566 of human RNF220 (NP_060620.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, Mouse spleen, Mouse thymus, Mouse brain, Rat testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 267-566 of human RNF220 (NP_060620.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | PKSL LLSA SIKR EGES PTAS PHSS ATDD LHHS DRYQ TFLR VRAN RQTR LNAR IGKM KRRK QDEG QREG SCMA EDDA VDIE HENN NRFE EYEW CGQK RIRA TTLL EGGF RGSG FIMC SGKE NPDS DADL DVDG DDTL EYGK PQYT EADV IPCT GEEP GEAK EREA LRGA VLNG GPPS TRIT PEFS KWAS DEMP STSN GESS KQEA MQKT CKNS DIEK ITED SAVT TFEA LKAR VREL ERQL SRGD RYKC LICM DSYS MPLT SIQC WHVH CEEC WLRT LGAK KLCP QCNT ITAP GDLR RIYL |
| Gene ID: | 55182 |
| Uniprot: | Q5VTB9 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 39kDa/62kDa |
| Observed MW: | 75kDa |
| Synonyms: | RNF220, C1orf164 |
| Background: |
| UniProt Code: | Q5VTB9 |
| NCBI GenInfo Identifier: | 46397375 |
| NCBI Gene ID: | 55182 |
| NCBI Accession: | NP_060620.2 |
| UniProt Related Accession: | Q5VTB9 |
| Molecular Weight: | Calculated: 39kDa; 62kDaObserved: 75kDa |
| NCBI Full Name: | E3 ubiquitin-protein ligase RNF220 isoform 1 |
| NCBI Synonym Full Names: | ring finger protein 220 |
| NCBI Official Symbol: | RNF220 |
| NCBI Official Synonym Symbols: | C1orf164 |
| NCBI Protein Information: | E3 ubiquitin-protein ligase RNF220 |
| UniProt Protein Name: | E3 ubiquitin-protein ligase RNF220 |
| UniProt Synonym Protein Names: | RING finger protein 220 |
| Protein Family: | E3 ubiquitin-protein ligase |
| UniProt Gene Name: | RNF220 |
| UniProt Entry Name: | RN220_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RNF220 antibody (CAB15853) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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