Cell Biology Antibodies 8

Anti-RNF212 Antibody (CAB3569)

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SKU:
CAB3569
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-RNF212 Antibody
Antibody SKU:CAB3569
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-297 of human RNF212 (NP_001124506.1).
Application:WB
Recommended Dilution:WB 1:200 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:THP-1, Mouse pancreas, Rat testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-297 of human RNF212 (NP_001124506.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MANW VFCN RCFQ PPHR TSCF SLTN CGHV YCDA CLGK GKKN ECLI CKAP CRTV LLSK HTDA DIQA FFMS IDSL CKKY SRET SQIL EFQE KHRK RLLA FYRE KISR LEES LRKS VLQI EQLQ SMRS SQQT AFST IKSS VSTK PHGC LLPP HSSA PDRL ESME VDLS PSPI RKSE IAAG PARI SMIS PPQD GRMG PHLT ASFC FIPW LTLS KPPV PGEC VISR GSPC FCID VCPH WLLL LAFS SGRH GELT NSKT LPIY AEVQ RAVL FPFQ QAEG TLDT FRTP AVSV VFPL CQFE RKKS F
Gene ID:285498
Uniprot:Q495C1
Cellular Location:Chromosome, Nucleus
Calculated MW:14kDa/21kDa/26kDa/30kDa/31kDa/33kDa
Observed MW:37kDa
Synonyms:RNF212, ZHP3
Background:This gene encodes a RING finger protein that may function as a ubiquitin ligase. The encoded protein may be involved in meiotic recombination. This gene is located within a linkage disequilibrium block and polymorphisms in this gene may influence recombination rates. Alternate splicing results in multiple transcript variants.
UniProt Protein Function:RNF212: SUMO E3 ligase that acts as a regulator of crossing-over during meiosis: required to couple chromosome synapsis to the formation of crossover-specific recombination complexes. Localizes to recombination sites and stabilizes meiosis-specific recombination factors, such as MutS-gamma complex proteins (MSH4 and MSH5) and TEX11. May mediate sumoylation of target proteins MSH4 and/or MSH5, leading to enhance their binding to recombination sites. Acts as a limiting factor for crossover designation and/or reinforcement and plays an antagonist role with CCNB1IP1/HEI10 in the regulation of meiotic recombination. 6 human isoforms produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 6.3.2.-; Ubiquitin conjugating system; SUMO ligase; Ligase

Chromosomal Location of Human Ortholog: 4p16.3

Cellular Component: synaptonemal complex

Molecular Function:zinc ion binding; ligase activity

Biological Process: protein sumoylation; meiotic recombination; meiotic gene conversion; chiasma formation

Disease: Recombination Rate Quantitative Trait Locus 1

NCBI Summary:This gene encodes a RING finger protein that may function as a ubiquitin ligase. The encoded protein may be involved in meiotic recombination. This gene is located within a linkage disequilibrium block and polymorphisms in this gene may influence recombination rates. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2010]
UniProt Code:Q495C1
NCBI GenInfo Identifier:121943260
NCBI Gene ID:285498
NCBI Accession:Q495C1.1
UniProt Secondary Accession:Q495C1,Q495C0, Q86W82, Q8IY99, Q8N8U7, C9J8N0,
UniProt Related Accession:Q495C1
Molecular Weight:297
NCBI Full Name:Probable E3 SUMO-protein ligase RNF212
NCBI Synonym Full Names:ring finger protein 212
NCBI Official Symbol:RNF212  
NCBI Official Synonym Symbols:ZHP3  
NCBI Protein Information:probable E3 SUMO-protein ligase RNF212
UniProt Protein Name:Probable E3 SUMO-protein ligase RNF212
UniProt Synonym Protein Names:RING finger protein 212
Protein Family:Probable E3 SUMO-protein ligase
UniProt Gene Name:RNF212  
UniProt Entry Name:RN212_HUMAN
Anti-RNF212 Antibody (CAB3569)Western blot analysis of extracts of various cell lines, using RNF212 antibody (CAB3569) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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