Cell Biology Antibodies 5
Anti-RNF181 Antibody (CAB14405)
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- SKU:
- CAB14405
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-RNF181 Antibody |
| Antibody SKU: | CAB14405 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-153 of human RNF181 (NP_057578.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | LO2, Mouse liver, Mouse brain, Mouse kidney, Mouse lung, Rat testis, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-153 of human RNF181 (NP_057578.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MASY FDEH DCEP SDPE QETR TNML LELA RSLF NRMD FEDL GLVV DWDH HLPP PAAK TVVE NLPR TVIR GSQA ELKC PVCL LEFE EEET AIEM PCHH LFHS SCIL PWLS KTNS CPLC RYEL PTDD DTYE EHRR DKAR KQQQ QHRL ENLH GAMY T |
| Gene ID: | 51255 |
| Uniprot: | Q9P0P0 |
| Cellular Location: | |
| Calculated MW: | 17kDa |
| Observed MW: | 18kDa |
| Synonyms: | RNF181, HSPC238 |
| Background: |
| UniProt Protein Function: | RNF181: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Belongs to the RNF181 family. |
| UniProt Protein Details: | Protein type:Ligase; Ubiquitin ligase; EC 6.3.2.-; Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 2p11.2 Molecular Function:zinc ion binding; ubiquitin-protein ligase activity; ligase activity Biological Process: protein autoubiquitination |
| NCBI Summary: | RNF181 binds the integrin alpha-IIb (ITGA2B; MIM 607759)/beta-3 (ITGB3; MIM 173470) complex and has E3 ubiquitin ligase activity (Brophy et al., 2008 [PubMed 18331836]).[supplied by OMIM, Dec 2008] |
| UniProt Code: | Q9P0P0 |
| NCBI GenInfo Identifier: | 74761852 |
| NCBI Gene ID: | 51255 |
| NCBI Accession: | Q9P0P0.1 |
| UniProt Related Accession: | Q9P0P0 |
| Molecular Weight: | 17kDa |
| NCBI Full Name: | E3 ubiquitin-protein ligase RNF181 |
| NCBI Synonym Full Names: | ring finger protein 181 |
| NCBI Official Symbol: | RNF181 |
| NCBI Official Synonym Symbols: | HSPC238 |
| NCBI Protein Information: | E3 ubiquitin-protein ligase RNF181 |
| UniProt Protein Name: | E3 ubiquitin-protein ligase RNF181 |
| UniProt Synonym Protein Names: | RING finger protein 181 |
| Protein Family: | E3 ubiquitin-protein ligase |
| UniProt Gene Name: | RNF181 |
| UniProt Entry Name: | RN181_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RNF181 antibody (CAB14405) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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