Signal Transduction Antibodies 2

Anti-RNF180 Antibody (CAB15241)

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SKU:
CAB15241
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-RNF180 Antibody
Antibody SKU:CAB15241
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-416 of human RNF180 (NP_848627.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:Jurkat, mouse brain, mouse testis, rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-416 of human RNF180 (NP_848627.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MKRS KELI TKNH SQEE TSIL RCWK CRKC IASS GCFM EYLE NQVI KDKD DSVD AQNI CHVW HMNV EALP EWIS CLIQ KAQW TVGK LNCP FCGA RLGG FNFV STPK CSCG QLAA VHLS KSRT DYQP TQAG RLMR PSVK YLSH PRVQ SGCD KEAL LTGG GSEN RNHR LLNM ARNN NDPG RLTE ALCL EVRP TYFE MKNE KLLS KASE PKYQ LFVP QLVT GRCA TRAF HRKS HSLD LNIS EKLT LLPT LYEI HSKT TAYS RLNE TQPI DLSG LPLQ SSKN SYSF QNPS SFDP SMLL QRFS VAPH ETQT QRGG EFQC GLEA ASVY SDHT NTNN LTFL MDLP SAGR SMPE ASDQ EEHL SPLD FLHS ANFS LGSI NQRL NKRE RSKL KNLR RKQR RRER WLQK QGKY SGVG LLDH MVSI YLLI
Gene ID:285671
Uniprot:Q86T96
Cellular Location:Endoplasmic reticulum membrane, Nucleus envelope, Single-pass membrane protein
Calculated MW:11kDa/47kDa/68kDa
Observed MW:65kDa
Synonyms:RNF180, RINES
Background:
UniProt Protein Function:RNF180: E3 ubiquitin-protein ligase which promotes polyubiquitination and degradation by the proteasome pathway of ZIC2. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Ligase; EC 6.3.2.-; Ubiquitin conjugating system; Ubiquitin ligase; Membrane protein, integral; EC 6.3.2.19

Chromosomal Location of Human Ortholog: 5q12.3

Cellular Component: intrinsic to endoplasmic reticulum membrane

Molecular Function:ubiquitin conjugating enzyme binding

Biological Process: adult behavior; norepinephrine metabolic process; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; protein polyubiquitination; serotonin metabolic process

UniProt Code:Q86T96
NCBI GenInfo Identifier:118573800
NCBI Gene ID:285671
NCBI Accession:Q86T96.2
UniProt Secondary Accession:Q86T96,Q0JSU3, Q495A8, Q8NBD1,
UniProt Related Accession:Q86T96
Molecular Weight:11,132 Da
NCBI Full Name:E3 ubiquitin-protein ligase RNF180
NCBI Synonym Full Names:ring finger protein 180
NCBI Official Symbol:RNF180  
NCBI Official Synonym Symbols:RINES  
NCBI Protein Information:E3 ubiquitin-protein ligase RNF180
UniProt Protein Name:E3 ubiquitin-protein ligase RNF180
UniProt Synonym Protein Names:RING finger protein 180
Protein Family:E3 ubiquitin-protein ligase
UniProt Gene Name:RNF180  
UniProt Entry Name:RN180_HUMAN
Anti-RNF180 Antibody (CAB15241)Western blot analysis of extracts of various cell lines, using RNF180 antibody (CAB15241) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
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