Cell Death Antibodies 1
Anti-RNF152 Antibody (CAB16608)
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- SKU:
- CAB16608
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Death
Description
| Antibody Name: | Anti-RNF152 Antibody |
| Antibody SKU: | CAB16608 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100-200 of human RNF152 (NP_775828.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | U-251MG, 293T, LO2 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100-200 of human RNF152 (NP_775828.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | YMLP LPIS KERA LLPG DMGC RLLP GSQQ KSVT VVTI PAEQ QPLQ GGAP QEAV EEEQ DRRG VVKS STWS GVCT VILV ACVL VFLL GIVL HNMS CISK RFTV I |
| Gene ID: | 220441 |
| Uniprot: | Q8N8N0 |
| Cellular Location: | Lysosome membrane, Single-pass membrane protein |
| Calculated MW: | 22kDa |
| Observed MW: | 22kDa |
| Synonyms: | RNF152 |
| Background: |
| UniProt Protein Function: | RNF152: E3 ubiquitin-protein ligase mediating 'Lys-48'-linked polyubiquitination of target proteins and their subsequent targeting to the proteasome for degradation. Induces apoptosis when overexpressed. Belongs to the RNF152 family. |
| UniProt Protein Details: | Protein type:Ubiquitin conjugating system; Ubiquitin ligase; EC 6.3.2.19; EC 6.3.2.-; Membrane protein, integral Chromosomal Location of Human Ortholog: 18q21.33 Cellular Component: lysosomal membrane; lysosome Molecular Function:ligase activity; small GTPase binding; ubiquitin-protein ligase activity; zinc ion binding Biological Process: apoptosis; cellular response to amino acid starvation; positive regulation of autophagy |
| UniProt Code: | Q8N8N0 |
| NCBI GenInfo Identifier: | 27734873 |
| NCBI Gene ID: | 220441 |
| NCBI Accession: | NP_775828.1 |
| UniProt Secondary Accession: | Q8N8N0,Q52LA4, B3KV99, |
| UniProt Related Accession: | Q8N8N0 |
| Molecular Weight: | 22,357 Da |
| NCBI Full Name: | E3 ubiquitin-protein ligase RNF152 |
| NCBI Synonym Full Names: | ring finger protein 152 |
| NCBI Official Symbol: | RNF152 |
| NCBI Protein Information: | E3 ubiquitin-protein ligase RNF152 |
| UniProt Protein Name: | E3 ubiquitin-protein ligase RNF152 |
| UniProt Synonym Protein Names: | RING finger protein 152 |
| Protein Family: | E3 ubiquitin-protein ligase |
| UniProt Gene Name: | RNF152 |
| UniProt Entry Name: | RN152_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RNF152 antibody (CAB16608) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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