Signal Transduction Antibodies 2
Anti-RNF149 Antibody (CAB15627)
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- SKU:
- CAB15627
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Description
| Antibody Name: | Anti-RNF149 Antibody |
| Antibody SKU: | CAB15627 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 221-400 of human RNF149 (NP_775918.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, A-549, mouse pancreas, rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 221-400 of human RNF149 (NP_775918.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | FYYI QRFL YTGS QIGS QSHR KETK KVIG QLLL HTVK HGEK GIDV DAEN CAVC IENF KVKD IIRI LPCK HIFH RICI DPWL LDHR TCPM CKLD VIKA LGYW GEPG DVQE MPAP ESPP GRDP AANL SLAL PDDD GSDD SSPP SASP AESE PQCD PSFK GDAG ENTA LLEA GRSD SRHG GPIS |
| Gene ID: | 284996 |
| Uniprot: | Q8NC42 |
| Cellular Location: | |
| Calculated MW: | 43kDa |
| Observed MW: | 43kDa |
| Synonyms: | RNF149, DNAPTP2 |
| Background: |
| UniProt Protein Function: | RNF149: E3 ubiquitin-protein ligase. Ubiquitinates BRAF, inducing its proteasomal degradation. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Ubiquitin ligase; EC 6.3.2.19; Ubiquitin conjugating system; Ligase; EC 6.3.2.- Chromosomal Location of Human Ortholog: 2q11.2 Cellular Component: membrane |
| UniProt Code: | Q8NC42 |
| NCBI GenInfo Identifier: | 160332298 |
| NCBI Gene ID: | 284996 |
| NCBI Accession: | Q8NC42.2 |
| UniProt Secondary Accession: | Q8NC42,Q53S14, Q8N5I8, Q8NBY5, Q8WUU3, |
| UniProt Related Accession: | Q8NC42 |
| Molecular Weight: | 43,165 Da |
| NCBI Full Name: | E3 ubiquitin-protein ligase RNF149 |
| NCBI Synonym Full Names: | ring finger protein 149 |
| NCBI Official Symbol: | RNF149 |
| NCBI Official Synonym Symbols: | DNAPTP2 |
| NCBI Protein Information: | E3 ubiquitin-protein ligase RNF149 |
| UniProt Protein Name: | E3 ubiquitin-protein ligase RNF149 |
| UniProt Synonym Protein Names: | DNA polymerase-transactivated protein 2; RING finger protein 149 |
| Protein Family: | E3 ubiquitin-protein ligase |
| UniProt Gene Name: | RNF149 |
| UniProt Entry Name: | RN149_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RNF149 antibody (CAB15627) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded rat brain using RNF149 antibody (CAB15627) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human stomach using RNF149 antibody (CAB15627) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse kidney using RNF149 antibody (CAB15627) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of C6 cells using RNF149 Polyclonal Antibody (CAB15627) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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