Cell Death Antibodies 1

Anti-RNF144B Antibody (CAB13723)

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SKU:
CAB13723
Product Type:
Antibody
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death

Description

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Antibody Name:Anti-RNF144B Antibody
Antibody SKU:CAB13723
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human RNF144B (NP_877434.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse, Rat
Positive Samples:Mouse kidney, Rat liver,
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human RNF144B (NP_877434.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MGSA GRLH YLAM TAEN PTPG DLAP APLI TCKL CLCE QSLD KMTT LQEC QCIF CTAC LKQY MQLA IREG CGSP ITCP DMVC LNHG TLQE AEIA CLVP VDQF QLYQ RLKF EREV HLDP YRTW CPVA DCQT VCPV ASSD PGQP VLVE CPSC HLKF CSCC KDAW HAEV SCRD SQPI VLPT EHRA LFGT DAEA PIKQ CPVC RVYI
Gene ID:255488
Uniprot:Q7Z419
Cellular Location:Cytoplasm, Mitochondrion membrane, Single-pass membrane protein
Calculated MW:23kDa/33kDa
Observed MW:34kDa
Synonyms:RNF144B, IBRDC2, PIR2, bA528A10.3, p53RFP
Background:
UniProt Protein Function:RNF144B: E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a p53/TP53-dependent but caspase-independent mechanism. However, its overexpression also produces a decrease of the ubiquitin-dependent stability of BAX, a pro-apoptotic protein, ultimately leading to protection of cell death; But, it is not an anti-apoptotic protein per se. Belongs to the RBR family. RNF144 subfamily.
UniProt Protein Details:

Protein type:EC 6.3.2.19; Ligase; Ubiquitin conjugating system; Ubiquitin ligase; Membrane protein, integral; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 6p22.3

Cellular Component: cytoplasm; cytosol; mitochondrial membrane; ubiquitin ligase complex

Molecular Function:protein binding; ubiquitin conjugating enzyme binding; ubiquitin-protein ligase activity

Biological Process: positive regulation of proteasomal ubiquitin-dependent protein catabolic process; protein polyubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process

UniProt Code:Q7Z419
NCBI GenInfo Identifier:57012811
NCBI Gene ID:255488
NCBI Accession:Q7Z419.1
UniProt Secondary Accession:Q7Z419,Q5TB85, Q6P4Q0, Q8N3R7, Q9BX38, B3KUA8, B4DZI2
UniProt Related Accession:Q7Z419
Molecular Weight:23,856 Da
NCBI Full Name:E3 ubiquitin-protein ligase RNF144B
NCBI Synonym Full Names:ring finger protein 144B
NCBI Official Symbol:RNF144B  
NCBI Official Synonym Symbols:PIR2; IBRDC2; p53RFP; bA528A10.3  
NCBI Protein Information:E3 ubiquitin-protein ligase RNF144B
UniProt Protein Name:E3 ubiquitin-protein ligase RNF144B
UniProt Synonym Protein Names:IBR domain-containing protein 2; RING finger protein 144B; p53-inducible RING finger protein
Protein Family:E3 ubiquitin-protein ligase
UniProt Gene Name:RNF144B  
UniProt Entry Name:R144B_HUMAN
Anti-RNF144B Antibody (CAB13723)Western blot analysis of extracts of various cell lines, using RNF144B antibody (CAB13723) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
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