Immunology Antibodies 1

Anti-RNF125 Antibody (CAB15166)

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SKU:
CAB15166
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

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Antibody Name:Anti-RNF125 Antibody
Antibody SKU:CAB15166
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-105 of human RNF125 (NP_060301.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:Raji, K-562
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-105 of human RNF125 (NP_060301.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MGSV LSTD SGKS APAS ATAR ALER RRDP ELPV TSFD CAVC LEVL HQPV RTRC GHVF CRSC IATS LKNN KWTC PYCR AYLP SEGV PATD VAKR MKSE YKNC AECD T
Gene ID:54941
Uniprot:Q96EQ8
Cellular Location:
Calculated MW:26kDa
Observed MW:28kDa
Synonyms:RNF125, TNORS, TRAC-1, TRAC1
Background:This gene encodes a novel E3 ubiquitin ligase that contains a RING finger domain in the N-terminus and three zinc-binding and one ubiquitin-interacting motif in the C-terminus. As a result of myristoylation, this protein associates with membranes and is primarily localized to intracellular membrane systems. The encoded protein may function as a positive regulator in the T-cell receptor signaling pathway.
UniProt Protein Function:RNF125: E3 ubiquitin-protein ligase that acts as a positive regulator of T-cell activation. E3 ligase proteins mediate ubiquitination and subsequent proteasomal degradation of target proteins.
UniProt Protein Details:

Protein type:Ubiquitin conjugating system; Ubiquitin ligase; EC 6.3.2.19; EC 6.3.2.-; Ligase

Chromosomal Location of Human Ortholog: 18q12.1

Molecular Function:zinc ion binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: protein ubiquitination; innate immune response; negative regulation of interferon type I production

Disease: Tenorio Syndrome

NCBI Summary:This gene encodes a novel E3 ubiquitin ligase that contains a RING finger domain in the N-terminus and three zinc-binding and one ubiquitin-interacting motif in the C-terminus. As a result of myristoylation, this protein associates with membranes and is primarily localized to intracellular membrane systems. The encoded protein may function as a positive regulator in the T-cell receptor signaling pathway. [provided by RefSeq, Mar 2012]
UniProt Code:Q96EQ8
NCBI GenInfo Identifier:37595555
NCBI Gene ID:54941
NCBI Accession:NP_060301
UniProt Related Accession:Q96EQ8
Molecular Weight:26kDa
NCBI Full Name:E3 ubiquitin-protein ligase RNF125
NCBI Synonym Full Names:ring finger protein 125
NCBI Official Symbol:RNF125  
NCBI Official Synonym Symbols:TNORS; TRAC1; TRAC-1  
NCBI Protein Information:E3 ubiquitin-protein ligase RNF125
UniProt Protein Name:E3 ubiquitin-protein ligase RNF125
UniProt Synonym Protein Names:RING finger protein 125; T-cell RING activation protein 1
Protein Family:E3 ubiquitin-protein ligase
UniProt Gene Name:RNF125  
UniProt Entry Name:RN125_HUMAN
Anti-RNF125 Antibody (CAB15166)Western blot analysis of extracts of various cell lines, using RNF125 antibody (CAB15166) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.
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