Neuroscience
Anti-RNF112 Antibody (CAB15333)
- SKU:
- CAB15333
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Neuroscience
Description
| Antibody Name: | Anti-RNF112 Antibody |
| Antibody SKU: | CAB15333 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 200-500 of human RNF112 (NP_009079.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:200 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 200-500 of human RNF112 (NP_009079.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | EGGR PRGG EASL QGCR WGAN GLAR GIWM WSHP FLLG KEGK KVAV FLVD TGDA MSPE LSRE TRIK LCAL TTML SSYQ ILST SQEL KDTD LDYL EMFV HVAE VMGK HYGM VPIQ HLDL LVRD SSHP NKAG QGHV GNIF QRLS GRYP KVQE LLQG KRAR CCLL PAPG RRRM NQGH ASPG DTDD DFRH LLGA YVSD VLSA APQH AKSR CQGY WNEG RAVA RGDR RLLT GQQL AQEI KNLS GWMG RTGP GFTS PDEM AAQL HDLR KVEA AKRE FEEY VRQQ DVAT KRIF SALR VLPD TMRN LLST Q |
| Gene ID: | 7732 |
| Uniprot: | Q9ULX5 |
| Cellular Location: | Membrane, Multi-pass membrane protein |
| Calculated MW: | 68kDa |
| Observed MW: | 68KDa |
| Synonyms: | RNF112, BFP, ZNF179 |
| Background: | This gene encodes a member of the RING finger protein family of transcription factors. The protein is primarily expressed in brain. The gene is located within the Smith-Magenis syndrome region on chromosome 17. |
| UniProt Protein Function: | RNF112: 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Hydrolase; Ubiquitin conjugating system; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 17p11.2 Cellular Component: cytoplasm; integral to membrane; nucleus Molecular Function:GTPase activity; GTP binding; zinc ion binding Biological Process: metabolic process; positive regulation of glial cell differentiation; positive regulation of neuron differentiation |
| NCBI Summary: | This gene encodes a member of the RING finger protein family of transcription factors. The protein is primarily expressed in brain. The gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9ULX5 |
| NCBI GenInfo Identifier: | 378405197 |
| NCBI Gene ID: | 7732 |
| NCBI Accession: | Q9ULX5.2 |
| UniProt Secondary Accession: | Q9ULX5,O60633, Q7Z5V9, |
| UniProt Related Accession: | Q9ULX5 |
| Molecular Weight: | 632 |
| NCBI Full Name: | RING finger protein 112 |
| NCBI Synonym Full Names: | ring finger protein 112 |
| NCBI Official Symbol: | RNF112 |
| NCBI Official Synonym Symbols: | BFP; ZNF179 |
| NCBI Protein Information: | RING finger protein 112; brain finger protein; zinc finger protein 179 |
| UniProt Protein Name: | RING finger protein 112 |
| UniProt Synonym Protein Names: | Brain finger protein; Zinc finger protein 179 |
| UniProt Gene Name: | RNF112 |
| UniProt Entry Name: | RN112_HUMAN |
 | Western blot analysis of extracts of Rat kidney, using RNF112 antibody (CAB15333) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunofluorescence analysis of C6 cells using RNF112 antibody (CAB15333) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of L929 cells using RNF112 antibody (CAB15333) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using RNF112 antibody (CAB15333) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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