Cell Biology Antibodies 18

Anti-RNF111 Antibody (CAB18277)

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SKU:
CAB18277
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-RNF111 Antibody
Antibody SKU:CAB18277
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant protein of human RNF111.
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:HepG2, SGC-7901, HeLa
Immunogen:Recombinant protein of human RNF111.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:54778
Uniprot:Q6ZNA4
Cellular Location:
Calculated MW:108kDa
Observed MW:120kDa
Anti-RNF111 Antibody (CAB18277)Western blot - RNF111 Polyclonal Antibody (CAB18277)
Synonyms:ARK, hRNF111, RNF111
Background:The protein encoded by this gene is a nuclear RING-domain containing E3 ubiquitin ligase. This protein interacts with the transforming growth factor (TGF) -beta/NODAL signaling pathway by promoting the ubiquitination and proteosomal degradation of negative regulators, like SMAD proteins, and thereby enhances TGF-beta target-gene transcription. As a modulator of the nodal signaling cascade, this gene plays a critical role in the induction of mesoderm during embryonic development. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.
UniProt Protein Function:RNF111: Acts in the NODAL pathway of mesoderm patterning during embryonic development. Acts downstream AXIN1 as an E3 ubiquitin- protein ligase which promotes the ubiquitination of inhibitory SMADs such as SMAD7, induces their proteasomal degradation and thereby enhances the transcriptional activity of TGF-beta and BMP. Activates Smad3/Smad4-dependent transcription by triggering signal-induced SnoN degradation. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Ligase; Ubiquitin ligase; EC 6.3.2.-; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 15q21

Cellular Component: cytoplasm; nucleolus; nucleoplasm; nucleus

Molecular Function:protein binding; SUMO polymer binding

Biological Process: positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of transforming growth factor beta receptor signaling pathway; proteasomal ubiquitin-dependent protein catabolic process; protein polyubiquitination; protein ubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process; ubiquitin-dependent SMAD protein catabolic process

NCBI Summary:The protein encoded by this gene is a nuclear RING-domain containing E3 ubiquitin ligase. This protein interacts with the transforming growth factor (TGF) -beta/NODAL signaling pathway by promoting the ubiquitination and proteosomal degradation of negative regulators, like SMAD proteins, and thereby enhances TGF-beta target-gene transcription. As a modulator of the nodal signaling cascade, this gene plays a critical role in the induction of mesoderm during embryonic development. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2012]
UniProt Code:Q6ZNA4
NCBI GenInfo Identifier:308153555
NCBI Gene ID:54778
NCBI Accession:Q6ZNA4.3
UniProt Secondary Accession:Q6ZNA4,Q6P9A4, Q6ZMU2, Q7L428, Q7Z346, Q8N1P9, Q8WUA3 Q9NSR1, C9JUS4, H0YN55,
UniProt Related Accession:Q6ZNA4
Molecular Weight:108,906 Da
NCBI Full Name:E3 ubiquitin-protein ligase Arkadia
NCBI Synonym Full Names:ring finger protein 111
NCBI Official Symbol:RNF111  
NCBI Official Synonym Symbols:ARK  
NCBI Protein Information:E3 ubiquitin-protein ligase Arkadia
UniProt Protein Name:E3 ubiquitin-protein ligase Arkadia
UniProt Synonym Protein Names:RING finger protein 111
Protein Family:E3 ubiquitin-protein ligase
UniProt Gene Name:RNF111  
UniProt Entry Name:RN111_HUMAN
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