Cell Biology Antibodies 2

Anti-RND3 Antibody (CAB11637)

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SKU:
CAB11637
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-RND3 Antibody
Antibody SKU:CAB11637
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 145-244 of human RND3 (NP_005159.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:293T
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 145-244 of human RND3 (NP_005159.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:TLVE LSNH RQTP VSYD QGAN MAKQ IGAA TYIE CSAL QSEN SVRD IFHV ATLA CVNK TNKN VKRN KSQR ATKR ISHM PSRP ELSA VATD LRKD KAKS CTVM
Gene ID:390
Uniprot:P61587
Cellular Location:Golgi apparatus membrane, Peripheral membrane protein
Calculated MW:27kDa
Observed MW:27kDa
Synonyms:RND3, ARHE, Rho8, RhoE, memB
Background:This gene encodes a protein which is a member of the small GTPase protein superfamily. The encoded protein binds only GTP but has no GTPase activity, and appears to act as a negative regulator of cytoskeletal organization leading to loss of adhesion. Multiple alternatively spliced variants, encoding the same protein, have been identified.
UniProt Protein Function:RhoE: Binds GTP but lacks intrinsic GTPase activity and is resistant to Rho-specific GTPase-activating proteins. Binds ROCK1. Interacts with UBXD5. Ubiquitous. Belongs to the small GTPase superfamily. Rho family.
UniProt Protein Details:

Protein type:G protein, monomeric; G protein, monomeric, Rho; G protein; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q23.3

Cellular Component: Golgi membrane; focal adhesion

Molecular Function:GTPase activity; protein binding; GTP binding

Biological Process: metabolic process; small GTPase mediated signal transduction; cell adhesion; actin cytoskeleton organization and biogenesis

NCBI Summary:This gene encodes a protein which is a member of the small GTPase protein superfamily. The encoded protein binds only GTP but has no GTPase activity, and appears to act as a negative regulator of cytoskeletal organization leading to loss of adhesion. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Dec 2011]
UniProt Code:P61587
NCBI GenInfo Identifier:47606459
NCBI Gene ID:390
NCBI Accession:P61587.1
UniProt Secondary Accession:P61587,P52199, D3DP95,
UniProt Related Accession:P61587
Molecular Weight:244
NCBI Full Name:Rho-related GTP-binding protein RhoE
NCBI Synonym Full Names:Rho family GTPase 3
NCBI Official Symbol:RND3  
NCBI Official Synonym Symbols:ARHE; Rho8; RhoE; memB  
NCBI Protein Information:rho-related GTP-binding protein RhoE; protein MemB; small GTP binding protein Rho8; ras homolog gene family, member E; rho-related GTP-binding protein Rho8
UniProt Protein Name:Rho-related GTP-binding protein RhoE
UniProt Synonym Protein Names:Protein MemB; Rho family GTPase 3; Rho-related GTP-binding protein Rho8; Rnd3
Protein Family:Rho-related GTP-binding protein
UniProt Gene Name:RND3  
UniProt Entry Name:RND3_HUMAN
Anti-RND3 Antibody (CAB11637)Western blot analysis of extracts of 293T cells, using RND3 antibody (CAB11637) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
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