Name: Anti-RNASEH2C Antibody (CAB13884)
Brand: Epigenetics & Nuclear Signaling Antibodies 1
SKU: CAB13884
Availability: OutOfStock

Antibody Name:	Anti-RNASEH2C Antibody
Antibody SKU:	CAB13884
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-164 of human RNASEH2C (NP_115569.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human
Positive Samples:	293T, HeLa

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-164 of human RNASEH2C (NP_115569.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MESG DEAA IERH RVHL RSAT LRDA VPAT LHLL PCEV AVDG PAPV GRFF TPAI RQGP EGLE VSFR GRCL RGEE VAVP PGLV GYVM VTEE KKVS MGKP DPLR DSGT DDQE EEPL ERDF DRFI GATA NFSR FTLW GLET IPGP DAKV RGAL TWPS LAAA IHAQ VPED
Gene ID:	84153
Uniprot:	Q8TDP1
Cellular Location:	Nucleus
Calculated MW:	17kDa
Observed MW:	20kDa

Synonyms:	RNASEH2C, AGS3, AYP1
Background:	This gene encodes a ribonuclease H subunit that can cleave ribonucleotides from RNA:DNA duplexes. Mutations in this gene cause Aicardi-Goutieres syndrome-3, a disease that causes severe neurologic dysfunction. A pseudogene for this gene has been identified on chromosome Y, near the sex determining region Y (SRY) gene.

UniProt Protein Function:	RNASEH2C: Non catalytic subunit of RNase H2, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging- strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes. Defects in RNASEH2C are the cause of Aicardi-Goutieres syndrome type 3 (AGS3). A form of Aicardi-Goutieres syndrome, a genetically heterogeneous disease characterized by cerebral atrophy, leukoencephalopathy, intracranial calcifications, chronic cerebrospinal fluid (CSF) lymphocytosis, increased CSF alpha-interferon, and negative serologic investigations for common prenatal infection. Clinical features as thrombocytopenia, hepatosplenomegaly and elevated hepatic transaminases along with intermittent fever may erroneously suggest an infective process. Severe neurological dysfunctions manifest in infancy as progressive microcephaly, spasticity, dystonic posturing and profound psychomotor retardation. Death often occurs in early childhood. Belongs to the RNase H2 subunit C family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:DNA replication Chromosomal Location of Human Ortholog: 11q13.1 Molecular Function:ribonuclease H activity Biological Process: RNA catabolic process Disease: Aicardi-goutieres Syndrome 3
NCBI Summary:	This gene encodes a ribonuclease H subunit that can cleave ribonucleotides from RNA:DNA duplexes. Mutations in this gene cause Aicardi-Goutieres syndrome-3, a disease that causes severe neurologic dysfunction. A pseudogene for this gene has been identified on chromosome Y, near the sex determining region Y (SRY) gene. [provided by RefSeq, Jul 2008]
UniProt Code:	Q8TDP1
NCBI GenInfo Identifier:	74730607
NCBI Gene ID:	84153
NCBI Accession:	Q8TDP1.1
UniProt Secondary Accession:	Q8TDP1,Q9H7F5,
UniProt Related Accession:	Q8TDP1
Molecular Weight:	17,712 Da
NCBI Full Name:	Ribonuclease H2 subunit C
NCBI Synonym Full Names:	ribonuclease H2 subunit C
NCBI Official Symbol:	RNASEH2C
NCBI Official Synonym Symbols:	AGS3; AYP1
NCBI Protein Information:	ribonuclease H2 subunit C
UniProt Protein Name:	Ribonuclease H2 subunit C
UniProt Synonym Protein Names:	Aicardi-Goutieres syndrome 3 protein; AGS3; RNase H1 small subunit; Ribonuclease HI subunit C
Protein Family:	Ribonuclease
UniProt Gene Name:	RNASEH2C
UniProt Entry Name:	RNH2C_HUMAN

Western blot analysis of extracts of various cell lines, using RNASEH2C antibody (CAB13884) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.

Antibodies

RNASEH2C Antibody (PACO11861)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)