Cell Biology Antibodies 10
Anti-RNASE1 Antibody (CAB6202)
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- SKU:
- CAB6202
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-RNASE1 Antibody |
| Antibody SKU: | CAB6202 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 29-156 of human RNASE1 (NP_937875.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Mouse |
| Positive Samples: | Mouse pancreas |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 29-156 of human RNASE1 (NP_937875.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | KESR AKKF QRQH MDSD SSPS SSST YCNQ MMRR RNMT QGRC KPVN TFVH EPLV DVQN VCFQ EKVT CKNG QGNC YKSN SSMH ITDC RLTN GSRY PNCA YRTS PKER HIIV ACEG SPYV PVHF DASV EDST |
| Gene ID: | 6035 |
| Uniprot: | P07998 |
| Cellular Location: | Secreted |
| Calculated MW: | 17kDa |
| Observed MW: | 15kDa |
| Synonyms: | RNASE1, RAC1, RIB1, RNS1 |
| Background: | This gene encodes a member of the pancreatic-type of secretory ribonucleases, a subset of the ribonuclease A superfamily. The encoded endonuclease cleaves internal phosphodiester RNA bonds on the 3'-side of pyrimidine bases. It prefers poly(C) as a substrate and hydrolyzes 2', 3'-cyclic nucleotides, with a pH optimum near 8.0. The encoded protein is monomeric and more commonly acts to degrade ds-RNA over ss-RNA. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. |
| UniProt Protein Function: | RNASE1: Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA. Belongs to the pancreatic ribonuclease family. |
| UniProt Protein Details: | Protein type:Ribonuclease; Secreted; EC 3.1.27.5; Secreted, signal peptide Chromosomal Location of Human Ortholog: 14q11.2 Molecular Function:protein binding; nucleic acid binding; pancreatic ribonuclease activity |
| NCBI Summary: | This gene encodes a member of the pancreatic-type of secretory ribonucleases, a subset of the ribonuclease A superfamily. The encoded endonuclease cleaves internal phosphodiester RNA bonds on the 3'-side of pyrimidine bases. It prefers poly(C) as a substrate and hydrolyzes 2',3'-cyclic nucleotides, with a pH optimum near 8.0. The encoded protein is monomeric and more commonly acts to degrade ds-RNA over ss-RNA. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P07998 |
| NCBI GenInfo Identifier: | 1350818 |
| NCBI Gene ID: | 6035 |
| NCBI Accession: | P07998.4 |
| UniProt Secondary Accession: | P07998,Q16830, Q16869, Q1KHR2, Q6ICS5, Q9UCB4, Q9UCB5 B2R589, D3DS06, |
| UniProt Related Accession: | P07998 |
| Molecular Weight: | 17,644 Da |
| NCBI Full Name: | Ribonuclease pancreatic |
| NCBI Synonym Full Names: | ribonuclease, RNase A family, 1 (pancreatic) |
| NCBI Official Symbol: | RNASE1 |
| NCBI Official Synonym Symbols: | RAC1; RIB1; RNS1 |
| NCBI Protein Information: | ribonuclease pancreatic; RIB-1; RNase 1; RNase A; HP-RNase; RNase upI-1; ribonuclease 1; ribonuclease A C1 |
| UniProt Protein Name: | Ribonuclease pancreatic |
| UniProt Synonym Protein Names: | HP-RNase; RIB-1; RNase UpI-1; Ribonuclease 1; RNase 1; Ribonuclease A; RNase A |
| Protein Family: | Ribonuclease |
| UniProt Gene Name: | RNASE1 |
| UniProt Entry Name: | RNAS1_HUMAN |
 | Western blot analysis of extracts of mouse pancreas, using RNASE1 antibody (CAB6202) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
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