Antibody Name:	Anti-RLN2 Antibody
Antibody SKU:	CAB6969
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 25-185 of human RLN2 (NP_604390.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Rat
Positive Samples:	22Rv1

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 25-185 of human RLN2 (NP_604390.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	DSWM EEVI KLCG RELV RAQI AICG MSTW SKRS LSQE DAPQ TPRP VAEI VPSF INKD TETI NMMS EFVA NLPQ ELKL TLSE MQPA LPQL QQHV PVLK DSSL LFEE FKKL IRNR QSEA ADSS PSEL KYLG LDTH SRKK RQLY SALA NKCC HVGC TKRS LARF C
Gene ID:	6019
Uniprot:	P04090
Cellular Location:	Secreted
Calculated MW:	12kDa/21kDa
Observed MW:	21kDa

Synonyms:

RLN2, H2, H2-RLX, RLXH2, bA12D24.1.1, bA12D24.1.2, relaxin 2

Background:

This gene encodes a member of the relaxin subfamily and insulin superfamily of peptide hormones. In humans there are three non-allelic relaxin genes. This gene encodes multiple protein isoforms, at least one of which undergoes proteolytic processing. This processing generates relaxin A and B chains that are linked by disulfide bonds to form the mature peptide hormone. This hormone plays a role in the male and female reproductive systems and was initially noted for its role in pregnancy. This protein also plays broader roles in the cardiovascular system, including in the regulation of blood pressure and control of heart rate, and data from animal models shows that this protein may have anti-fibrotic and cardioprotective effects.

UniProt Protein Function:	RLN2: Relaxin is an ovarian hormone that acts with estrogen to produce dilatation of the birth canal in many mammals. May be involved in remodeling of connective tissues during pregnancy, promoting growth of pubic ligaments and ripening of the cervix. Belongs to the insulin family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 9p24.1 Cellular Component: extracellular region Molecular Function:hormone activity Biological Process: female pregnancy
NCBI Summary:	Relaxins are known endocrine and autocrine/paracrine hormones, belonging to the insulin gene superfamily. In humans there are three non-allelic relaxin genes, RLN1, RLN2 and RLN3, where RLN1 and RLN2 share high sequence homology. The active form of the protein encoded by this gene consists of an A chain and a B chain linked by disulfide bonds. Relaxin is produced by the ovary, and targets the mammalian reproductive system to ripen the cervix, elongate the pubic symphysis and inhibit uterine contraction. It may have additional roles in enhancing sperm motility, regulating blood pressure, controlling heart rate and releasing oxytocin and vasopressin. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2013]
UniProt Code:	P04090
NCBI GenInfo Identifier:	132281
NCBI Gene ID:	6019
NCBI Accession:	P04090.1
UniProt Secondary Accession:	P04090,Q99936, Q9UCX3, Q9UQJ2, A0AVM0,
UniProt Related Accession:	P04090
Molecular Weight:	185
NCBI Full Name:	Prorelaxin H2
NCBI Synonym Full Names:	relaxin 2
NCBI Official Symbol:	RLN2
NCBI Official Synonym Symbols:	H2; RLXH2; H2-RLX; bA12D24.1.1; bA12D24.1.2
NCBI Protein Information:	prorelaxin H2; H2-preprorelaxin; relaxin, ovarian, of pregnancy
UniProt Protein Name:	Prorelaxin H2
Protein Family:	Prorelaxin
UniProt Gene Name:	RLN2
UniProt Entry Name:	REL2_HUMAN

	Western blot analysis of extracts of 22Rv1 cells, using RLN2 antibody (CAB6969) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat kidney using RLN2 antibody (CAB6969) at dilution of 1:200 (40x lens).