Name: Anti-RLIM Antibody (CAB15837)
Brand: Epigenetics & Nuclear Signaling Antibodies 2
SKU: CAB15837
Availability: OutOfStock

Antibody Name:	Anti-RLIM Antibody
Antibody SKU:	CAB15837
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 535-624 of human RLIM (NP_057204.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	Raji, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 535-624 of human RLIM (NP_057204.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	FLLN EDDD DQPR GLTK EQID NLAM RSFG ENDA LKTC SVCI TEYT EGNK LRKL PCSH EYHV HCID RWLS ENST CPIC RRAV LASG NRES VV
Gene ID:	51132
Uniprot:	Q9NVW2
Cellular Location:	Nucleus
Calculated MW:	52kDa/68kDa
Observed MW:	75kDa

Synonyms:

RLIM, MRX61, NY-REN-43, RNF12

Background:

The protein encoded by this gene is a RING-H2 zinc finger protein. It has been shown to be an E3 ubiquitin protein ligase that targets LIM domain binding 1 (LDB1/CLIM), and causes proteasome-dependent degradation of LDB1. This protein and LDB1 are co-repressors of LHX1/LIM-1, a homeodomain transcription factor. Multiple alternatively spliced variants, encoding the same protein, have been identified.

UniProt Protein Function:	RNF12: E3 ubiquitin-protein ligase. Acts as a negative coregulator for LIM homeodomain transcription factors by mediating the ubiquitination and subsequent degradation of LIM cofactors LDB1 and LDB2 and by mediating the recruitment the SIN3a/histone deacetylase corepressor complex. Ubiquitination and degradation of LIM cofactors LDB1 and LDB2 allows DNA-bound LIM homeodomain transcription factors to interact with other protein partners such as RLIM. Plays a role in telomere length-mediated growth suppression by mediating the ubiquitination and degradation of TERF1. By targeting ZFP42 for degradation, acts as an activator of random inactivation of X chromosome in the embryo, a stochastic process in which one X chromosome is inactivated to minimize sex- related dosage differences of X-encoded genes in somatic cells of female placental mammals. Belongs to the RNF12 family.
UniProt Protein Details:	Protein type:Ubiquitin ligase; Ubiquitin conjugating system; Ligase; EC 6.3.2.-; Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: Xq13-q21 Cellular Component: nucleoplasm; transcriptional repressor complex; cytoplasm; nucleus Molecular Function:zinc ion binding; ubiquitin-protein ligase activity; transcription corepressor activity; ligase activity Biological Process: ubiquitin-dependent protein catabolic process; transcription, DNA-dependent; negative regulation of transcription factor activity; protein ubiquitination; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent
NCBI Summary:	The protein encoded by this gene is a RING-H2 zinc finger protein. It has been shown to be an E3 ubiquitin protein ligase that targets LIM domain binding 1 (LDB1/CLIM), and causes proteasome-dependent degradation of LDB1. This protein and LDB1 are co-repressors of LHX1/LIM-1, a homeodomain transcription factor. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Feb 2009]
UniProt Code:	Q9NVW2
NCBI GenInfo Identifier:	143811451
NCBI Gene ID:	51132
NCBI Accession:	Q9NVW2.3
UniProt Related Accession:	Q9NVW2
Molecular Weight:	Calculated: 52kDa; 68kDaObserved: 75kDa
NCBI Full Name:	E3 ubiquitin-protein ligase RLIM
NCBI Synonym Full Names:	ring finger protein, LIM domain interacting
NCBI Official Symbol:	RLIM
NCBI Official Synonym Symbols:	MRX61; RNF12; TOKAS; NY-REN-43
NCBI Protein Information:	E3 ubiquitin-protein ligase RLIM
UniProt Protein Name:	E3 ubiquitin-protein ligase RLIM
UniProt Synonym Protein Names:	LIM domain-interacting RING finger protein; RING finger LIM domain-binding protein; R-LIM; RING finger protein 12; Renal carcinoma antigen NY-REN-43
Protein Family:	E3 ubiquitin-protein ligase
UniProt Gene Name:	RLIM
UniProt Entry Name:	RNF12_HUMAN

Western blot analysis of extracts of various cell lines, using RLIM antibody (CAB15837) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 150s.

Antibodies

RLIM Antibody (PACO11854)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)