Signal Transduction Antibodies 2
Anti-RIT1 Antibody (CAB15715)
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- SKU:
- CAB15715
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Description
| Antibody Name: | Anti-RIT1 Antibody |
| Antibody SKU: | CAB15715 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human RIT1 (NP_001243749.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Mouse, Rat |
| Positive Samples: | Mouse kidnay, Rat kdney |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human RIT1 (NP_001243749.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | SDLK QLRQ VTKE EGLA LARE FSCP FFET SAAY RYYI DDVF HALV REIR RKEK EAVL AMEK KSKP KNSV WKRL KSPF RKKK DSVT |
| Gene ID: | 6016 |
| Uniprot: | Q92963 |
| Cellular Location: | Cell membrane |
| Calculated MW: | 21kDa/25kDa/27kDa |
| Observed MW: | 30kDa |
| Synonyms: | RIT1, NS8, RIBB, RIT, ROC1 |
| Background: | This gene encodes a member of a subfamily of Ras-related GTPases. The encoded protein is involved in regulating p38 MAPK-dependent signaling cascades related to cellular stress. This protein also cooperates with nerve growth factor to promote neuronal development and regeneration. Alternate splicing results in multiple transcript variants. |
| UniProt Protein Function: | RIT1: a small G protein of the Ras family. Plays a crucial role in coupling NGF stimulation to the activation of both EPHB2 and p38-alpha signaling pathways and in NGF- dependent neuronal differentiation. Stimulation of the NGF and EGF receptor signaling pathways results in rapid and prolonged activation. Interacts with afadin, the C-terminal domain of RalGDS and RLF, but not with RIN1 and PIK3CA. RLF binds exclusively to the active GTP-bound form. Strongly interacts with BRAF, but only weakly with c-Raf. BRAF and c-RAF association is dependent upon the GTP-bound state. Interacts with RGL3. Shows rapid uncatalyzed guanine nucleotide dissociation rates, which are much faster than those of most Ras subfamily members. |
| UniProt Protein Details: | Protein type:G protein, monomeric, Ras; G protein; G protein, monomeric Chromosomal Location of Human Ortholog: 1q22 Molecular Function:calmodulin binding; protein binding Biological Process: Ras protein signal transduction; signal transduction Disease: Noonan Syndrome 8 |
| NCBI Summary: | This gene encodes a member of a subfamily of Ras-related GTPases. The encoded protein is involved in regulating p38 MAPK-dependent signaling cascades related to cellular stress. This protein also cooperates with nerve growth factor to promote neuronal development and regeneration. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012] |
| UniProt Code: | Q92963 |
| NCBI GenInfo Identifier: | 38258628 |
| NCBI Gene ID: | 6016 |
| NCBI Accession: | Q92963.1 |
| UniProt Secondary Accession: | Q92963,O00646, O00720, Q5VY89, Q5VY90, B4DQE8, |
| UniProt Related Accession: | Q92963 |
| Molecular Weight: | 27,148 Da |
| NCBI Full Name: | GTP-binding protein Rit1 |
| NCBI Synonym Full Names: | Ras like without CAAX 1 |
| NCBI Official Symbol: | RIT1 |
| NCBI Official Synonym Symbols: | NS8; RIT; RIBB; ROC1 |
| NCBI Protein Information: | GTP-binding protein Rit1 |
| UniProt Protein Name: | GTP-binding protein Rit1 |
| UniProt Synonym Protein Names: | Ras-like protein expressed in many tissues; Ras-like without CAAX protein 1 |
| Protein Family: | GTP-binding protein |
| UniProt Gene Name: | RIT1 |
| UniProt Entry Name: | RIT1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RIT1 antibody (CAB15715) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
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