Cell Death Antibodies 2
Anti-RIPK3 Antibody (CAB5431)
- SKU:
- CAB5431
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Death
Description
| Antibody Name: | Anti-RIPK3 Antibody |
| Antibody SKU: | CAB5431 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human RIPK3 (NP_006862.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HT-29 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human RIPK3 (NP_006862.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSCV KLWP SGAP APLV SIEE LENQ ELVG KGGF GTVF RAQH RKWG YDVA VKIV NSKA ISRE VKAM ASLD NEFV LRLE GVIE KVNW DQDP KPAL VTKF MENG SLSG LLQS QCPR PWPL LCRL |
| Gene ID: | 11035 |
| Uniprot: | Q9Y572 |
| Cellular Location: | Cell membrane, Cytoplasm, Mitochondrion, cytosol |
| Calculated MW: | 25kDa/27kDa/56kDa |
| Observed MW: | 57KDa |
| Synonyms: | RIPK3, RIP3 |
| Background: | The product of this gene is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, and contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor. |
| UniProt Protein Function: | RIPK3: Promotes apoptosis. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Binds TRAF2 and RIPK1 and is recruited to the TNFR-1 signaling complex. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; Kinase, protein; TKL group; RIPK family Chromosomal Location of Human Ortholog: 14q11.2 Cellular Component: mitochondrion; plasma membrane; cytosol Molecular Function:NF-kappaB-inducing kinase activity; protein serine/threonine kinase activity; identical protein binding; protein binding; transcription coactivator activity; protein complex binding; ATP binding; protein kinase activity Biological Process: spleen development; I-kappaB kinase/NF-kappaB cascade; regulation of activated T cell proliferation; thymus development; protein heterooligomerization; protein amino acid autophosphorylation; MyD88-independent toll-like receptor signaling pathway; T cell differentiation in the thymus; protein modification process; toll-like receptor 3 signaling pathway; signal transduction; lymph node development; activation of NF-kappaB transcription factor; regulation of T cell mediated cytotoxicity; regulation of adaptive immune response; regulation of interferon-gamma production; toll-like receptor signaling pathway; positive regulation of interferon type I production; activation of protein kinase activity; innate immune response; T cell homeostasis; positive regulation of ligase activity; toll-like receptor 4 signaling pathway; protein homooligomerization; positive regulation of oxidoreductase activity |
| UniProt Code: | Q9Y572 |
| NCBI GenInfo Identifier: | 40254844 |
| NCBI Gene ID: | 11035 |
| NCBI Accession: | NP_006862 |
| UniProt Secondary Accession: | Q9Y572,Q5J795, Q5J796, Q6P5Y1, B4DJL9, C4AM87, |
| UniProt Related Accession: | Q9Y572 |
| Molecular Weight: | 25,326 Da |
| NCBI Full Name: | receptor-interacting serine/threonine-protein kinase 3 |
| NCBI Synonym Full Names: | receptor-interacting serine-threonine kinase 3 |
| NCBI Official Symbol: | RIPK3 |
| NCBI Official Synonym Symbols: | RIP3 |
| NCBI Protein Information: | receptor-interacting serine/threonine-protein kinase 3; RIP-3; RIP-like protein kinase 3; receptor interacting protein 3; receptor-interacting protein 3 |
| UniProt Protein Name: | Receptor-interacting serine/threonine-protein kinase 3 |
| UniProt Synonym Protein Names: | RIP-like protein kinase 3; Receptor-interacting protein 3; RIP-3 |
| Protein Family: | Putative zinc metalloprotease |
| UniProt Gene Name: | RIPK3 |
| UniProt Entry Name: | RIPK3_HUMAN |
 | Western blot analysis of extracts of HT-29 cells, using RIPK3 Rabbit pAb (CAB5431) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded human liver cancer using RIPK3 antibody (CAB5431) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse pancreas using RIPK3 antibody (CAB5431) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of C6 cells using RIPK3 antibody (CAB5431) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of L929 cells using RIPK3 antibody (CAB5431) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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