Name: Anti-RIPK1 Antibody (CAB7414)
Brand: Cell Death Antibodies 2
SKU: CAB7414
Availability: OutOfStock

Antibody Name:	Anti-RIPK1 Antibody
Antibody SKU:	CAB7414
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 170-440 of human RIPK1 (NP_003795.2).

Application:	WB IHC IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Jurkat, HT-1080, DU145, K-562, NIH/3T3, Mouse liver, C6

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 170-440 of human RIPK1 (NP_003795.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	NEEH NELR EVDG TAKK NGGT LYYM APEH LNDV NAKP TEKS DVYS FAVV LWAI FANK EPYE NAIC EQQL IMCI KSGN RPDV DDIT EYCP REII SLMK LCWE ANPE ARPT FPGI EEKF RPFY LSQL EESV EEDV KSLK KEYS NENA VVKR MQSL QLDC VAVP SSRS NSAT EQPG SLHS SQGL GMGP VEES WFAP SLEH PQEE NEPS LQSK LQDE ANYH LYGS RMDR QTKQ QPRQ NVAY NREE ERRR RVSH DPFA QQRP YENF QNTE GKGT AYS
Gene ID:	8737
Uniprot:	Q13546
Cellular Location:	Cell membrane, Cytoplasm
Calculated MW:	70kDa/75kDa
Observed MW:	76kDa

Synonyms:	RIP, RIP-1, RIP1, RIPK1
Background:

UniProt Protein Function:	RIPK1: Serine-threonine kinase which transduces inflammatory and cell-death signals (necroptosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage. Upon activation of TNFR1 by the TNF-alpha family cytokines, TRADD and TRAF2 are recruited to the receptor. Ubiquitination by TRAF2 via 'Lys-63'-link chains acts as a critical enhancer of communication with downstream signal transducers in the mitogen-activated protein kinase pathway and the NF-kappa-B pathway, which in turn mediate downstream events including the activation of genes encoding inflammatory molecules. Polyubiquitinated protein binds to IKBKG/NEMO, the regulatory subunit of the IKK complex, a critical event for NF-kappa-B activation. Interaction with other cellular RHIM-containing adapters initiates gene activation and cell death. RIPK1 and RIPK3 association, in particular, forms a necroptosis-inducing complex. Interacts (via RIP homotypic interaction motif) with RIPK3 (via RIP homotypic interaction motif); this interaction induces RIPK1 necroptosis-specific phosphorylation, formation of the necroptosis-inducing complex. Interacts (via the death domain) with TNFRSF6 (via the death domain) and TRADD (via the death domain). Is recruited by TRADD to TNFRSF1A in a TNF-dependent process. Binds RNF216, EGFR, IKBKG, TRAF1, TRAF2 and TRAF3. Interacts with BNLF1. Interacts with SQSTM1 upon TNF-alpha stimulation. May interact with MAVS/IPS1. Interacts with ZFAND5. Interacts with RBCK1. Interacts with BIRC2/c-IAP1, BIRC3/c-IAP2 and XIAP/BIRC4. Inhibited by necrostatin-1. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; Protein kinase, TKL; TKL group; RIPK family Chromosomal Location of Human Ortholog: 6p25.2 Cellular Component: mitochondrion; cytoplasm; cytosol; receptor complex; lipid raft Molecular Function:identical protein binding; protein serine/threonine kinase activity; protein binding; ubiquitin protein ligase binding; death receptor binding; protein complex binding; ATP binding; protein kinase activity Biological Process: caspase activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; protein heterooligomerization; positive regulation of apoptosis; apoptosis; MyD88-independent toll-like receptor signaling pathway; protein amino acid autophosphorylation; positive regulation of JNK cascade; negative regulation of I-kappaB kinase/NF-kappaB cascade; toll-like receptor 3 signaling pathway; positive regulation of tumor necrosis factor production; activation of JNK activity; activation of NF-kappaB transcription factor; positive regulation of interleukin-8 production; positive regulation of programmed cell death; tumor necrosis factor-mediated signaling pathway; cellular protein catabolic process; toll-like receptor signaling pathway; positive regulation of interferon type I production; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; positive regulation of macrophage differentiation; toll-like receptor 4 signaling pathway; protein homooligomerization
NCBI Summary:	This gene encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein plays a role in inflammation and cell death in response to tissue damage, pathogen recognition, and as part of developmental regulation. RIPK1/RIPK3 kinase-mediated necrosis is referred to as necroptosis. Genetic disruption of this gene in mice results in death shortly after birth. [provided by RefSeq, Aug 2017]
UniProt Code:	Q13546
NCBI GenInfo Identifier:	57242761
NCBI Gene ID:	8737
NCBI Accession:	NP_003795.2
UniProt Secondary Accession:	Q13546,Q13180, Q59H33, A0AV89, B2RAG1, B4E3F9,
UniProt Related Accession:	Q13546
Molecular Weight:	75931 MW
NCBI Full Name:	receptor-interacting serine/threonine-protein kinase 1 isoform 1
NCBI Synonym Full Names:	receptor interacting serine/threonine kinase 1
NCBI Official Symbol:	RIPK1
NCBI Official Synonym Symbols:	RIP; RIP1; RIP-1
NCBI Protein Information:	receptor-interacting serine/threonine-protein kinase 1
UniProt Protein Name:	Receptor-interacting serine/threonine-protein kinase 1
UniProt Synonym Protein Names:	Cell death protein RIP; Receptor-interacting protein 1; RIP-1; Serine/threonine-protein kinase RIP
Protein Family:	Receptor-interacting serine/threonine-protein kinase
UniProt Gene Name:	RIPK1
UniProt Entry Name:	RIPK1_HUMAN

	Western blot analysis of extracts of various cell lines, using RIPK1 antibody (CAB7414) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Western blot analysis of extracts of various cell lines, using RIPK1 antibody (CAB7414) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded human tonsil using RIPK1 antibody (CAB7414) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat lung using RIPK1 antibody (CAB7414) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse spleen using RIPK1 antibody (CAB7414) at dilution of 1:100 (40x lens).